Combinatorial Surface Roughness Effects on Osteoclastogenesis and Osteogenesis
| Autor: | Yang Zhang, Jeroen J. J. P. van den Beucken, Jinlong Shao, S. Elisa Chen |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
musculoskeletal diseases
0301 basic medicine Bone Regeneration Materials science Anabolism Surface Properties Cytoskeletal Organization Osteoclasts osteogenic differentiation Biocompatible Materials Bone Marrow Cells 02 engineering and technology subtype Mice 03 medical and health sciences Osteogenesis Osteoclast medicine Surface roughness Animals Humans General Materials Science Bone Resorption Bone regeneration Cell Nucleus Titanium Osteoblasts Gene Expression Profiling Macrophages Stem Cells Bone implant RANK Ligand Cell Differentiation DNA 021001 nanoscience & nanotechnology Phenotype Cell biology RAW 264.7 Cells 030104 developmental biology medicine.anatomical_structure coupling of bone resorption and formation Cell culture Culture Media Conditioned surface roughness 0210 nano-technology Research Article |
| Zdroj: | ACS Applied Materials & Interfaces |
| ISSN: | 1944-8252 1944-8244 |
| DOI: | 10.1021/acsami.8b10992 |
| Popis: | Implant surface properties are a key factor in bone responses to metallic bone implants. In view of the emerging evidence on the important role of osteoclasts in bone regeneration, we here studied how surface roughness affects osteoclastic differentiation and to what extent these osteoclasts have stimulatory effects on osteogenic differentiation of osteoprogenitor cells. For this, we induced osteoclasts derived from RAW264.7 cell line and primary mouse macrophages on titanium surfaces with different roughness (Ra 0.02–3.63 μm) and analyzed osteoclast behavior in terms of cell number, morphology, differentiation, and further anabolic effect on osteoblastic cells. Surfaces with different roughness induced the formation of osteoclasts with distinct phenotypes, based on total osteoclast numbers, morphology, size, cytoskeletal organization, nuclearity, and osteoclastic features. Furthermore, these different osteoclast phenotypes displayed differential anabolic effects toward the osteogenic differentiation of osteoblastic cells, for which the clastokine CTHRC1 was identified as a causative factor. Morphologically, osteoclast potency to stimulate osteogenic differentiation of osteoblastic cells was found to logarithmically correlate with the nuclei number per osteoclast. Our results demonstrate the existence of a combinatorial effect of surface roughness, osteoclastogenesis, and osteogenic differentiation. These insights open up a new dimension for designing and producing metallic implants by considering the implant roughness to locally regulate osseointegration through coupling osteoclastogenesis with osteogenesis. |
| Databáze: | OpenAIRE |
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