The enigma of microtubule coils in brain synaptosomes
| Autor: | Richard Cumming, L. E. Westrum, Robert D. Burgoyne, Edward George Gray, Julie Barron |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Rana temporaria
chemistry.chemical_element macromolecular substances Calcium Biology Microtubules Synaptic vesicle chemistry.chemical_compound Microtubule Tannic acid Animals Cytoskeleton General Environmental Science Synaptosome urogenital system General Engineering Dendrites Axons Rats Microscopy Electron chemistry Biochemistry Synapses Biophysics Krebs solution General Earth and Planetary Sciences Synaptic Vesicles Veratridine Synaptosomes |
| Zdroj: | Proceedings of the Royal Society of London. Series B. Biological Sciences. 216:385-396 |
| ISSN: | 2053-9193 0080-4649 |
| DOI: | 10.1098/rspb.1982.0082 |
| Popis: | When synaptosomes are prepared from rat brain and incubated in Krebs solution, the presynaptic bulb develops a coil of microtubules (mts). Various considerations indicate that the coil does not have a cytoskeletal supportive function. Synaptosome coil mts show certain peculiarities, e. g. they thrive during incubation in Krebs solution (dendritic mts are depolymerized in Krebs solution) and they show no protofilament molecular substructure with tannic acid. Dendritic mts show clearly a 13 protofilament substructure when processed in the same way. Synaptosomal coil mts are sensitive to micromolar calcium and are depolymerized by treatment of the synaptosomes with veratridine or A23187. Our evidence indicates that coil mts of synaptosome and synaptic vesicle clothed mts of ‘intact’ albumin-treated synapses are different morphological and functional entities. As mentioned above, the function of coil mts remains enigmatic, while the mts seen in albumin-treated synapses could well have a role in synaptic vesicle translocation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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