Multiclass screening method to detect more than fifty banned substances in bovine bile and urine
| Autor: | Francesca Lega, Rosanna Rossi, Antimo Gioiello, Elisabetta Manuali, Lorenzo Rigoni, Roberta Galarini, Giorgio Saluti, Simone Moretti, Danilo Giusepponi |
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| Rok vydání: | 2018 |
| Předmět: |
Screening test
Urine Mass spectrometry 01 natural sciences Biochemistry Mass Spectrometry Analytical Chemistry Lactones chemistry.chemical_compound 0404 agricultural biotechnology Adrenal Cortex Hormones Stilbenes Screening method Animals Hypnotics and Sedatives Bile Environmental Chemistry Anabolic drugs Cattle Liquid-chromatography coupled to hybrid high-resolution mass spectrometry Mycotoxin Zearalenone Spectroscopy High rate Chromatography 010401 analytical chemistry 04 agricultural and veterinary sciences Adrenergic beta-Agonists 040401 food science 0104 chemical sciences Substance Abuse Detection Critical parameter chemistry Nitroimidazoles Steroids Progestins Chromatography Liquid |
| Zdroj: | Analytica Chimica Acta. 1032:56-67 |
| ISSN: | 0003-2670 |
| DOI: | 10.1016/j.aca.2018.06.037 |
| Popis: | A multiclass screening method to detect fifty-three forbidden substances by liquid-chromatography coupled to hybrid high-resolution mass spectrometry (LC-Q-Orbitrap) was developed and validated in bovine bile and urine. Eight classes of compounds were included in the method's scope (β-agonists, corticosteroids, nitroimidazoles, progestins, resorcylic acid lactones (RALs), sedatives, steroids and stilbenes) plus chloramphenicol and dapsone. After hydrolysis, the sample was divided in two aliquots, which followed two parallel purification steps. The reunified extracts were injected and two chromatographic runs performed in positive and negative ionization mode, respectively. The validation data (60 different samples per matrix) proved that the method was fit for purpose with detection capabilities lower than 1 μg L−1 in both matrices. The combined application of accurate mass acquisition and two-stage mass spectrometry (parallel reaction monitoring) was crucial to achieve suitable selectivity, which is the most critical parameter mainly for urines. Finally, the long-standing problem of the high rate of false positive results for RALs, due to the natural ingestion of mycotoxin, zearalenone, was taken on including all their labelled standards. That allowed a very satisfactory management of this screening test. |
| Databáze: | OpenAIRE |
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