GLI3 Is Stabilized by SPOP Mutations and Promotes Castration Resistance via Functional Cooperation with Androgen Receptor in Prostate Cancer
| Autor: | Chun Liang Chen, Tim H M Huang, Xiang Gu, Thu Minh Duong, Marieke Burleson, Haojie Huang, Yuqian Yan, Yi Zou, Michael A. Liss, Thomas G. Boyer, Lu-Zhe Sun, P. Renee Yew, Acarizia Easley, Addanki P. Kumar, Debodipta Das, Janice Jianhong Deng, Yi Chen, Tai Qin, Roble Bedolla |
|---|---|
| Rok vydání: | 2022 |
| Předmět: |
Male
Cancer Research animal structures medicine.drug_class Nerve Tissue Proteins SPOP Article Mice Prostate cancer Castration Resistance Zinc Finger Protein Gli3 Cell Line Tumor medicine Animals Humans Sonic hedgehog Molecular Biology biology business.industry Nuclear Proteins Ubiquitin-Protein Ligase Complexes Gene signature Androgen medicine.disease Repressor Proteins Androgen receptor Prostatic Neoplasms Castration-Resistant Oncology Receptors Androgen Mutation embryonic structures biology.protein Cancer research Signal transduction business |
| Zdroj: | Mol Cancer Res |
| ISSN: | 1557-3125 1541-7786 |
| Popis: | Although the Sonic hedgehog (SHH) signaling pathway has been implicated in promoting malignant phenotypes of prostate cancer, details on how it is activated and exerts its oncogenic role during prostate cancer development and progression is less clear. Here, we show that GLI3, a key SHH pathway effector, is transcriptionally upregulated during androgen deprivation and posttranslationally stabilized in prostate cancer cells by mutation of speckle-type POZ protein (SPOP). GLI3 is a substrate of SPOP-mediated proteasomal degradation in prostate cancer cells and prostate cancer driver mutations in SPOP abrogate GLI3 degradation. Functionally, GLI3 is necessary and sufficient for the growth and migration of androgen receptor (AR)–positive prostate cancer cells, particularly under androgen-depleted conditions. Importantly, we demonstrate that GLI3 physically interacts and functionally cooperates with AR to enrich an AR-dependent gene expression program leading to castration-resistant growth of xenografted prostate tumors. Finally, we identify an AR/GLI3 coregulated gene signature that is highly correlated with castration-resistant metastatic prostate cancer and predictive of disease recurrence. Together, these findings reveal that hyperactivated GLI3 promotes castration-resistant growth of prostate cancer and provide a rationale for therapeutic targeting of GLI3 in patients with castration-resistant prostate cancer (CRPC). Implications: We describe two clinically relevant mechanisms leading to hyperactivated GLI3 signaling and enhanced AR/GLI3 cross-talk, suggesting that GLI3-specific inhibitors might prove effective to block prostate cancer development or delay CRPC. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|