Frequency-Selective Rotation of Two-Particle Nanoactuators for Rapid and Sensitive Detection of Biomolecules
| Autor: | Andrea Ranzoni, Jjhb Jean Schleipen, Leo van Lj IJzendoorn, Mwj Menno Prins |
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| Přispěvatelé: | Molecular Biosensing for Med. Diagnostics |
| Rok vydání: | 2011 |
| Předmět: |
Time Factors
Light Metal Nanoparticles Nanoparticle Bioengineering Nanotechnology Biosensing Techniques Buffers Light scattering Magnetics Cluster Analysis Humans Scattering Radiation Light beam Nanobiotechnology General Materials Science Particle Size Dose-Response Relationship Drug Chemistry Scattering Mechanical Engineering General Chemistry equipment and supplies Condensed Matter Physics Chemical physics Nanoparticles Particle Magnetic nanoparticles human activities Biosensor |
| Zdroj: | Nano Letters, 11(5), 2017-2022. American Chemical Society |
| ISSN: | 1530-6992 1530-6984 |
| DOI: | 10.1021/nl200384p |
| Popis: | We describe an optomagnetic bionanotechnology for rapid and sensitive solution-based affinity assays. Nanoactuators made from bioactive magnetic nanoparticles undergo rotational motion in the volume of a fluid under frequency-controlled magnetic actuation. The nanoactuators show a time-dependent scattering cross-section to an incoming light beam. We demonstrate that the temporal behavior of the scattered light intensity relates to the number, the magnetic properties and the size distribution of the nanoactuators, independently revealing the average value and variation in the magnetic properties of the nanoparticles as well as the concentration of nanoactuators. The method is applied to detect biomolecules in fluid by interparticle binding. In a total assay time of less than 3 min, we demonstrate a limit of detection lower than 400 fM in buffer and 5 pM in human plasma. |
| Databáze: | OpenAIRE |
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