Trehalose-6-phosphate promotes fermentation and glucose repression in Saccharomyces cerevisiae

Autor: Rebeca L. Vicente, Sébastien Déjean, Jean-Luc Parrou, Lucie Spina, Jose P.L. Gómez, Jean Marie François
Přispěvatelé: Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP), Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Institut de Mathématiques de Toulouse UMR5219 (IMT), Université Toulouse Capitole (UT Capitole), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université Toulouse - Jean Jaurès (UT2J), Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), ANR Blanc [07-2-200101], Region Midi Pyrenees (France), Toulouse Region Midi Pyrenees, Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Recherche Agronomique (INRA), Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université Toulouse 1 Capitole (UT1), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Université Toulouse 1 Capitole (UT1)-Université Toulouse - Jean Jaurès (UT2J)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés ( LISBP ), Institut National de la Recherche Agronomique ( INRA ) -Institut National des Sciences Appliquées - Toulouse ( INSA Toulouse ), Institut National des Sciences Appliquées ( INSA ) -Institut National des Sciences Appliquées ( INSA ) -Centre National de la Recherche Scientifique ( CNRS ), Institut de Mathématiques de Toulouse UMR5219 ( IMT ), Université Toulouse 1 Capitole ( UT1 ) -Université Toulouse - Jean Jaurès ( UT2J ) -Université Toulouse III - Paul Sabatier ( UPS ), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-PRES Université de Toulouse-Institut National des Sciences Appliquées - Toulouse ( INSA Toulouse )
Jazyk: angličtina
Rok vydání: 2018
Předmět:
0301 basic medicine
trehalose 6-phosphate
Applied Microbiology
yeast
Applied Microbiology and Biotechnology
flux sensing
Glycolysis
Crabtree effect
lcsh:QH301-705.5
ComputingMilieux_MISCELLANEOUS
[SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology
chemistry.chemical_classification
biology
quantitative analysis
Chemistry
filamentous fungi
glycolysis
Complementation
filamentous fungus
Biochemistry
analyse quantitative
TPS1
glucose repression
profil métabolique
Autre (Sciences du Vivant)
Saccharomyces cerevisiae
Biochemistry
Genetics and Molecular Biology (miscellaneous)
Microbiology
03 medical and health sciences
Virology
capacité
Genetics
[ MATH.MATH-ST ] Mathematics [math]/Statistics [math.ST]
mutant
Molecular Biology
levure
Sugar phosphates
Ethanol
Wild type
Cell Biology
biology.organism_classification
Yeast
champignon filamenteux
030104 developmental biology
lcsh:Biology (General)
Fermentation
Parasitology
Zdroj: Microbial Cell
Microbial Cell, 2018, 5 (10), pp.444-459. ⟨10.15698/mic2018.10.651⟩
Microbial Cell 10 (5), 444-459. (2018)
Microbial Cell, Shared Science Publishers OG, 2018, 5 (10), pp.444-459. ⟨10.15698/mic2018.10.651⟩
Microbial Cell, Shared Science Publishers OG, 2018, 5 (10), pp.444-459. 〈10.15698/mic2018.10.651〉
Microbial Cell, Vol 5, Iss 10, Pp 444-459 (2018)
ISSN: 2311-2638
DOI: 10.15698/mic2018.10.651⟩
Popis: The yeast trehalose-6-phosphate synthase (Tps1) catalyzes the formation of trehalose-6-phosphate (T6P) in trehalose synthesis. Besides, Tps1 plays a key role in carbon and energy homeostasis in this microbial cell, as shown by the well documented loss of ATP and hyper accumulation of sugar phosphates in response to glucose addition in a mutant defective in this protein. The inability of a Saccharomyces cerevisiae tps1 mutant to cope with fermentable sugars is still a matter of debate. We reexamined this question through a quantitative analysis of the capability of TPS1 homologues from different origins to complement phenotypic defects of this mutant. Our results allowed to classify this complementation in three groups. A first group enclosed TPS1 of Klyveromyces lactis with that of S. cerevisiae as their expression in Sctps1 cells fully recovered wild type metabolic patterns and fermentation capacity in response to glucose. At the opposite was the group with TPS1 homologues from the bacteria Escherichia coli and Ralstonia solanacearum, the plant Arabidopsis thaliana and the insect Drosophila melanogaster whose metabolic profiles were comparable to those of a tps1 mutant, notably with almost no accumulation of T6P, strong impairment of ATP recovery and potent reduction of fermentation capacity, albeit these homologous genes were able to rescue growth of Sctps1 on glucose. In between was a group consisting of TPS1 homologues from other yeast species and filamentous fungi characterized by 5 to 10 times lower accumulation of T6P, a weaker recovery of ATP and a 3-times lower fermentation capacity than wild type. Finally, we found that glucose repression of gluconeogenic genes was strongly dependent on T6P. Altogether, our results suggest that the TPS protein is indispensable for growth on fermentable sugars, and points to a critical role of T6P as a sensing molecule that promotes sugar fermentation and glucose repression..
Databáze: OpenAIRE
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