Experimental hypoxic–ischemic encephalopathy: comparison of apparent diffusion coefficients and proton magnetic resonance spectroscopy
| Autor: | AiQun Cai, Yang-Kang Li, Guo-Rui Liu, Xiu-Guo Zhou |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Resuscitation
medicine.medical_specialty Magnetic Resonance Spectroscopy Time Factors Swine Biomedical Engineering Biophysics Brain damage Creatine Hypoxic Ischemic Encephalopathy Choline Cerebral edema Diffusion chemistry.chemical_compound immune system diseases Internal medicine mental disorders medicine Animals Humans Effective diffusion coefficient HSP70 Heat-Shock Proteins Radiology Nuclear Medicine and imaging business.industry Brain medicine.disease Immunohistochemistry nervous system diseases Hsp70 Endocrinology nervous system chemistry Creatinine Hypoxia-Ischemia Brain Lactates Protons medicine.symptom Nuclear medicine business |
| Zdroj: | Magnetic Resonance Imaging. 28:487-494 |
| ISSN: | 0730-725X |
| DOI: | 10.1016/j.mri.2009.12.002 |
| Popis: | This study aims to compare the apparent diffusion coefficients (ADCs) and proton magnetic resonance spectroscopy ((1)H-MRS) in the first 24 h of acute hypoxic-ischemic brain damage (HIBD) in piglets. Twenty-five 7-day-old piglets were subjected to transient bilateral common carotid artery occlusion followed by ventilation with 4% oxygen for 1 h. Diffusion-weighted imaging (DWI) and (1)H-MRS were performed on cessation of the insult or at 3, 6, 12 or 24 h after resuscitation (all n=5). ADCs, N-acetylaspartate/choline (NAA/Cho), NAA/creatine (NAA/Cr), lactate/NAA (Lac/NAA), Lac/Cho and Lac/Cr were calculated. Cerebral injury was evaluated by pathological study and Hsp70 immunohistochemical analysis. On cessation of the insult, ADCs, NAA/Cho and NAA/Cr reduced, Lac/NAA, Lac/Cho and Lac/Cr increased. From 3 to 12 h after resuscitation, ADCs, Lac/NAA, Lac/Cho and Lac/Cr recovered, NAA/Cho and NAA/Cr reduced. Twenty-four hours after resuscitation, ADCs reduced once more, Lac/NAA, Lac/Cho and Lac/Cr increased again, whereas NAA/Cho and NAA/Cr decreased continuously. Pathological study revealed mild cerebral edema on cessation of the insult and more and more severe cerebral injury after resuscitation. No Hsp70-positive cells were detected on cessation of the insult. From 3 to 12 hours after resuscitation, Hsp70-positive cells gradually increased. Twenty-four hours after resuscitation, Hsp70-positive cells decreased. Throughout the experiment, changes in NAA/Cho and pathology had the best correlation (R=-0.729). In conclusion, NAA/Cho is the most precise ratio to reflect the pathological changes of early HIBD. Transient ADCs and Lac ratios recovery do not predict the reversal of histological damage of early HIBD. Reducing astrocytic swelling is of great clinical significance. |
| Databáze: | OpenAIRE |
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