Cell Fate of Retinal Progenitor Cells: In Ovo UbC-StarTrack Analysis

Autor: Cindy Lorena Olmos Carreño, María Figueres-Oñate, Laura Lopez-Mascaraque, Gabriel Scicolone
Přispěvatelé: Ministerio de Ciencia e Innovación (España), Consejo Nacional de Investigaciones Científicas y Técnicas (Argentina), Universidad de Buenos Aires
Rok vydání: 2022
Předmět:
Zdroj: International Journal of Molecular Sciences; Volume 23; Issue 20; Pages: 12388
ISSN: 1422-0067
DOI: 10.3390/ijms232012388
Popis: Clonal cell analysis outlines the ontogenic potential of single progenitor cells, allowing the elucidation of the neural heterogeneity among different cell types and their lineages. In this work, we analyze the potency of retinal stem/progenitor cells through development using the chick embryo as a model. We implemented in ovo the clonal genetic tracing strategy UbC-StarTrack for tracking retinal cell lineages derived from individual progenitors of the ciliary margin at E3.5 (HH21-22). The clonal assignment of the derived-cell progeny was performed in the neural retina at E11.5-12 (HH38) through the identification of sibling cells as cells expressing the same combination of fluorophores. Moreover, cell types were assessed based on their cellular morphology and laminar location. Ciliary margin derived-cell progenies are organized in columnar associations distributed along the peripheral retina with a limited tangential dispersion. The analysis revealed that, at the early stages of development, this region harbors multipotent and committed progenitor cells.
This work was supported by the following grants: MCIN/AEI/10.13039/501100, funded by MCIN/AEI/10.13039/501100011033, Program Enhancing Mobility between Latin American and Caribbean countries and Europe (EMHE-20016), Consejo Nacional de Investigaciones Científicas y Técnicas (PIP 00441), Universidad de Buenos Aires (00526BA and 00769BA).
Databáze: OpenAIRE
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