Understanding the B and T cell epitopes of spike protein of severe acute respiratory syndrome coronavirus-2: A computational way to predict the immunogens
| Autor: | Vipin Jagrit, Yoya Vashi, Sachin Kumar |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Models Molecular 030106 microbiology Pneumonia Viral Epitopes T-Lymphocyte Gene Expression Peptide Human leukocyte antigen Plasma protein binding Genome Viral Biology Spike protein Microbiology Epitope Article 03 medical and health sciences Betacoronavirus Epitopes Protein structure HLA Antigens Genetics Humans Amino Acid Sequence Binding site Peptide sequence Molecular Biology Pandemics Diagnostics Ecology Evolution Behavior and Systematics chemistry.chemical_classification Binding Sites Immunodominant Epitopes SARS-CoV-2 COVID-19 Viral Vaccines Virology 030104 developmental biology Infectious Diseases chemistry Spike Glycoprotein Coronavirus Epitopes B-Lymphocyte Glycoprotein Coronavirus Infections Peptides Protein Binding |
| Zdroj: | Infection, Genetics and Evolution |
| ISSN: | 1567-7257 1567-1348 |
| Popis: | The 2019 novel severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) outbreak has caused a large number of deaths, with thousands of confirmed cases worldwide. The present study followed computational approaches to identify B- and T-cell epitopes for the spike (S) glycoprotein of SARS-CoV-2 by its interactions with the human leukocyte antigen alleles. We identified 24 peptide stretches on the SARS-CoV-2 S protein that are well conserved among the reported strains. The S protein structure further validated the presence of predicted peptides on the surface, of which 20 are surface exposed and predicted to have reasonable epitope binding efficiency. The work could be useful for understanding the immunodominant regions in the surface protein of SARS-CoV-2 and could potentially help in designing some peptide-based diagnostics. Also, identified T-cell epitopes might be considered for incorporation in vaccine designs. Highlights • Identification of the B- and T-cell epitopes for spike glycoprotein of SARS-CoV-2. • Interactions of B and T cell epitopes with the human leukocyte antigen alleles. |
| Databáze: | OpenAIRE |
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