Regeneration of Transected Recurrent Laryngeal Nerve Using Hybrid-Transplantation of Skeletal Muscle-Derived Stem Cells and Bioabsorbable Scaffold
Autor: | Daisuke Maki, Ippei Yamato, Soji Ozawa, Shuichi Soeda, Akihito Kazuno, Yoshiyasu Uchiyama, Tetsuro Tamaki, Nobuyuki Nakajima, Hiroya Seta |
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Rok vydání: | 2018 |
Předmět: |
vocal cord function
p75 N200 qPCR immunohistochemistry 0301 basic medicine Pathology medicine.medical_specialty lcsh:Medicine Article 03 medical and health sciences 0302 clinical medicine medicine Recurrent laryngeal nerve business.industry Regeneration (biology) lcsh:R General Medicine Transplantation 030104 developmental biology medicine.anatomical_structure Nerve growth factor Multipotent Stem Cell Endoneurium Stem cell business Perineurium 030217 neurology & neurosurgery |
Zdroj: | Journal of Clinical Medicine Journal of Clinical Medicine, Vol 7, Iss 9, p 276 (2018) Journal of Clinical Medicine; Volume 7; Issue 9; Pages: 276 |
ISSN: | 2077-0383 |
Popis: | Hybrid transplantation of skeletal muscle-derived multipotent stem cells (Sk-MSCs) and bioabsorbable polyglyconate (PGA) felt was studied as a novel regeneration therapy for the transected recurrent laryngeal nerve (RLN). Sk-MSCs were isolated from green fluorescence protein transgenic mice and then expanded and transplanted with PGA felt for the hybrid transplantation (HY group) into the RLN transected mouse model. Transplantation of culture medium (M group) and PGA + medium (PGA group) were examined as controls. After eight weeks, trans-oral video laryngoscopy demonstrated 80% recovery of spontaneous vocal-fold movement during breathing in the HY group, whereas the M and PGA groups showed wholly no recoveries. The Sk-MSCs showed active engraftment confined to the damaged RLN portion, representing favorable prevention of cell diffusion on PGA, with an enhanced expression of nerve growth factor mRNAs. Axonal re-connection in the HY group was confirmed by histological serial sections. Immunohistochemical analysis revealed the differentiation of Sk-MSCs into Schwann cells and perineurial/endoneurial cells and axonal growth supportive of perineurium/endoneurium. The number of axons recovered was over 86%. These results showed that the stem cell and cytokine delivery system using hybrid transplantation of Sk-MSCs/PGA-felt is a potentially practical and useful approach for the recovery of transected RLN. |
Databáze: | OpenAIRE |
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