Differential expression of LFA-3, Fas and MHC Class I on Ad5- and Ad12-transformed human cells and their susceptibility to lymphokine-activated killer (LAK) cells
| Autor: | Graham P. Cook, John Holt, Rob C. Hoeben, Graham Bottley, G. Eric Blair, Josephine L. Meade |
|---|---|
| Rok vydání: | 2005 |
| Předmět: |
viruses
Cell CD1 NK cells Adenoviridae Virology Cell Line Tumor MHC class I medicine Cytotoxic T cell Adenovirus Humans fas Receptor Adenovirus E1B Proteins Killer Cells Lymphokine-Activated LAK cells Cell Line Transformed DNA Primers Lymphokine-activated killer cell biology Base Sequence Reverse Transcriptase Polymerase Chain Reaction Histocompatibility Antigens Class I Lymphokine E1A E1B biochemical phenomena metabolism and nutrition Fas Natural killer T cell CD58 Antigens Cell biology stomatognathic diseases medicine.anatomical_structure Gene Expression Regulation Interleukin 12 biology.protein LFA-3 Adenovirus E1A Proteins |
| Zdroj: | Virology. 338(2) |
| ISSN: | 0042-6822 |
| Popis: | Adenovirus (Ad) E1A is a potent oncogene and has been shown to deregulate the expression of a large number of cellular genes leading to cellular transformation. Here we have analysed the expression of several immunomodulatory molecules on the surface of a set of human cell lines transformed with either Ad12 or Ad5. Human cells transformed with Ad12 demonstrated reduced expression of cell surface LFA-3, Fas and MHC class I when compared to Ad5-transformed cells. Furthermore, Ad12-transformed human cell lines demonstrated greater susceptibility to lysis by lymphokine-activated killer (LAK) cells, compared to Ad5-transformed human cell lines. In contrast, previous studies with rodent cells showed that both Ad5- and Ad12-transformed rat cells were susceptible to LAK cells. Thus, transformation of human cells with Ad5 or Ad12 results in differences in the expression of immunomodulatory molecules on the cell surface and differential recognition of these virus-transformed cells by immune effector cells. |
| Databáze: | OpenAIRE |
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