Engineering and repair of diaphragm using biosheet (a collagenous connective tissue membrane) in rabbits
| Autor: | Tetsuro Kodaka, Hiroko Komura, Makoto Komura, Keisuke Suzuki, Yasuhide Nakayama, Kan Terawaki, Takumi Gohara |
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| Rok vydání: | 2018 |
| Předmět: |
Dense connective tissue
Diaphragm Diaphragmatic breathing Connective tissue 030204 cardiovascular system & hematology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Silicone Tissue engineering Absorbable Implants Animals Medicine Polytetrafluoroethylene Tissue Engineering Guided Tissue Regeneration business.industry Congenital diaphragmatic hernia General Medicine Anatomy medicine.disease Diaphragm (structural system) Transplantation Treatment Outcome medicine.anatomical_structure chemistry Connective Tissue 030220 oncology & carcinogenesis Pediatrics Perinatology and Child Health Female Surgery Collagen Rabbits Hernias Diaphragmatic Congenital business |
| Zdroj: | Journal of Pediatric Surgery. 53:330-334 |
| ISSN: | 0022-3468 |
| Popis: | Background Prosthetic patches can be used to repair large congenital diaphragmatic hernia defects but may be associated with infection, recurrence, and thoracic deformity. Biosheets (collagenous connective tissue membranes) have been used in regenerative medicine. We evaluated the efficacy of Biosheets in a rabbit model. Methods Biosheets were prepared by embedding silicone plates in dorsal subcutaneous pouches of rabbits for 4weeks. In group 1 (n=11), Gore-Tex® sheets (1.8×1.8cm) were implanted into a diaphragmatic defect. In group 2 (n=11), Seamdura®, a bioabsorbable artificial dural substitute, was implanted in the same manner. In group 3 (n=14), biosheets were autologously transplanted into the diaphragmatic defects. All rabbits were euthanized 3months after transplantation to evaluate their graft status. Results Herniation of liver was observed in 5 rabbits (45%) in group 1, 8 (73%) in group 2, and 3 (21%) in group 3. A significant difference was noted between groups 2 and 3 (P=0.017). Biosheets had equivalent burst strength and modulus of elasticity as native diaphragm. Muscular tissue regeneration in transplanted biosheets in group 3 was confirmed histologically. Conclusion Biosheets may be applied to diaphragmatic repair and replacement of diaphragmatic muscular tissue. Level of evidence Level III. |
| Databáze: | OpenAIRE |
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