Dietary supplementation of alpha-linolenic acid in an enriched rapeseed oil diet protects from stroke

Autor: B. Delplanque, Nicolas Blondeau, C. Gandin, Jean-Louis Nahon, Carole Rovère, G. Agnani, N. Simon-Rousseau, Catherine Heurteaux, Carine Nguemeni
Přispěvatelé: Institut de pharmacologie moléculaire et cellulaire (IPMC), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Neuroendocrinologie Moléculaire de la Prise Alimentaire, Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA), ONIDOL, Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2009
Předmět:
Male
Linolenic acid
Physiology
[SDV.BC]Life Sciences [q-bio]/Cellular Biology
Biology
Neuroprotection
Lipid peroxidation
Fatty Acids
Monounsaturated
03 medical and health sciences
chemistry.chemical_compound
Mice
0302 clinical medicine
Nutraceutical
Functional food
Dietary Fats
Unsaturated
medicine
Animals
Plant Oils
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
Stroke
030304 developmental biology
2. Zero hunger
Pharmacology
chemistry.chemical_classification
0303 health sciences
Omega-3 polyunsaturated fatty acids
alpha-Linolenic acid
alpha-Linolenic Acid
[SDV.SP]Life Sciences [q-bio]/Pharmaceutical sciences
medicine.disease
3. Good health
Mice
Inbred C57BL
Experimental diet
chemistry
Biochemistry
Dietary Supplements
[SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]
[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie
Rapeseed Oil
Lipid Peroxidation
[SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
030217 neurology & neurosurgery
Polyunsaturated fatty acid
Zdroj: Pharmacological Research
Pharmacological Research, Elsevier, 2010, 61 (3), pp.226-33. ⟨10.1016/j.phrs.2009.12.007⟩
ISSN: 1096-1186
1043-6618
DOI: 10.1016/j.phrs.2009.12.007⟩
Popis: International audience; Populations of Western countries are severely deficient in omega-3 intake, both in the form of alpha-linolenic acid (ALA) and the Long Chain derivatives (LC-n-3), Eicosa-Pentaenoic-Acid and Docosa-Hexaenoic-Acid. Omega-3 insufficiency is a risk factor for cardiovascular and cerebral diseases such as coronary heart disease and stroke. Stroke is a major cause of mortality and morbidity, and induces a significant socioeconomic cost and a marked increase in patient/family burden. To date, preventive treatments and neuroprotective drugs identified in preclinical studies failed in clinical trials, in part because of an inability to tolerate drugs at neuroprotective concentrations. Therefore testing alternative protective strategies, such as functional foods/nutraceuticals, are of considerable interest. We have previously demonstrated that a single injection of ALA reduced ischemic damage by limiting glutamate-mediated neuronal death, whereas repeated injections displayed additive protective benefits as a result of increased neurogenesis, synaptogenesis and neurotrophin expression. Because intravenous injections are not a suitable long-term strategy in humans, the present study investigated the effect of ALA supplementation by an experimental diet containing rapeseed oil (RSO, a rich source of ALA) as the only source of lipids for stroke prevention. We tested several experimental diets which included 5, 10, and 20% RSO-enriched diet and feeding paradigms (fresh diet was provided once or twice a week for 4 or 6 weeks). Our results showed that ALA supplemented diets are more sensitive to lipid peroxidation than a regular chow diet. Because the diet affected feeding behavior and animal growth, we defined concrete guidelines to investigate the effect of omega-3 supplementation on neuropathology. Among the different sets of experiments, animals fed with 10% and 20% RSO-enriched diet displayed a reduced mortality rate, infarct size and increased probability of spontaneous reperfusion in the post-ischemic period. In addition, a drastic reduction of lipid peroxidation levels was observed in the ischemic brain of RSO-fed animals. Overall, our findings provide new insights into the potential of employing rapeseed oil as a functional food/nutraceutical aiding in stroke prevention and protection.
Databáze: OpenAIRE
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