Effects of aortic coarctation on aortic antioxidant enzymes and NADPH oxidase protein expression
| Autor: | Christian K. Roberts, Chang-De Zhan, Ashkan Ehdaie, Ram K. Sindhu, Nosratola D. Vaziri |
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| Rok vydání: | 2005 |
| Předmět: |
Male
medicine.medical_specialty Antioxidant medicine.medical_treatment Blotting Western medicine.disease_cause Antioxidants Aortic Coarctation General Biochemistry Genetics and Molecular Biology Nitric oxide Rats Sprague-Dawley Superoxide dismutase chemistry.chemical_compound medicine.artery Internal medicine medicine Animals Thoracic aorta General Pharmacology Toxicology and Pharmaceutics chemistry.chemical_classification Reactive oxygen species NADPH oxidase biology Superoxide Dismutase Glutathione peroxidase NADPH Oxidases General Medicine Catalase Rats Up-Regulation Disease Models Animal Endocrinology chemistry Biochemistry biology.protein Oxidative stress |
| Zdroj: | Life Sciences. 76:945-953 |
| ISSN: | 0024-3205 |
| DOI: | 10.1016/j.lfs.2004.10.014 |
| Popis: | Abdominal aortic coarctation above the renal arteries leads to severe hypertension above the stenotic site and provides a model for simultaneous testing of the effects of increased and decreased pressure and consequently shear stress in the same animal. The effects of increased pressure, per se, on oxidative stress and antioxidant enzyme expression is unknown. We studied the protein expressions of antioxidant enzymes and NADPH oxidase (gp91phox subunit) in the aortic segments above and below the stenosis site in sham-operated control and aortic-banded rats at four weeks postoperatively. Compared with the control group, the banded group showed significant up-regulation of NADPH oxidase, catalase (CAT), Cu/Zn superoxide dismutase (SOD) and Mn SOD protein content in the thoracic aorta. In contrast, Mn SOD, Cu/Zn SOD and NADPH oxidase protein abundance were unchanged in the abdominal aortic segment below the stricture where blood pressure is not elevated, whereas CAT protein abundance was also elevated in the abdominal aorta. No changes were noted for glutathione peroxidase (GPX) protein content either in the thoracic or abdominal aortic segments. Coarctation-induced hypertension is associated with increased aortic CAT, Cu/Zn SOD, Mn SOD and NADPH oxidase protein expression. The up-regulation of NADPH oxidase increases reactive oxygen species (ROS) generation noted in the present study and contributes to inactivation of nitric oxide (NO) as shown previously in this model. Upregulation of antioxidant enzymes may be a compensatory response in the face of elevated pressure and oxidative stress. The normality of protein abundance in the abdominal aorta wherein blood pressure is not elevated points to the role of baromechanical factors, as opposed to circulating humoral factors that were similar in both segments, as a mechanism responsible for increased antioxidant enzyme expression. |
| Databáze: | OpenAIRE |
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