TPN DECREASES IL-4 AND IL-10 mRNA EXPRESSION IN LIPOPOLYSACCHARIDE STIMULATED INTESTINAL LAMINA PROPRIA CELLS BUT GLUTAMINE SUPPLEMENTATION PRESERVES THE EXPRESSION
| Autor: | Ben L. Zarzaur, Kazuhiko Fukatsu, M K Hanna, R C DeWitt, Yong Wu, K A Kudsk |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Lipopolysaccharides
Male medicine.medical_specialty Lipopolysaccharide Lymphoid Tissue Glutamine medicine.medical_treatment Enzyme-Linked Immunosorbent Assay Biology Critical Care and Intensive Care Medicine Enteral administration Mice chemistry.chemical_compound Internal medicine Intestine Small Weight Loss medicine Animals RNA Messenger Intestinal Mucosa Interleukin 4 Gastrostomy Laparotomy Mice Inbred ICR Lamina propria Reverse Transcriptase Polymerase Chain Reaction Interleukin Animal Feed Immunoglobulin A Interleukin-10 Interleukin 10 medicine.anatomical_structure Endocrinology Cytokine Gene Expression Regulation chemistry Immunology Emergency Medicine Cytokines Parenteral Nutrition Total Interleukin-4 |
| Zdroj: | Shock. 15:318-322 |
| ISSN: | 1073-2322 |
| DOI: | 10.1097/00024382-200115040-00012 |
| Popis: | Total parenteral nutrition (TPN) decreases intestinal IgA and levels of Th2 cytokines, interleukin (IL)-4, and IL-10 within the supernatants of intestinal homogenates. These cytokines are known to stimulate IgA production in vitro by cells of the gut-associated lymphoid tissue (GALT). Glutamine (GLN) supplementation of TPN normalizes GALT mass and cytokine levels. Because intestinal homogenates contain mucosa which itself is a source of cytokines, it was unclear whether cytokines change within the GALT itself. This study investigates dietary effects on IL-4 and IL-10 cytokine mRNA expression within isolated GALT lamina propria cells after lipopolysaccharide (LPS) stimulation. Prospective randomized experimental trials were used in this study. Fifty-nine mice were randomized to chow, intravenous TPN (IV-TPN), intragastric TPN (IG-TPN), complex enteral diet (CED), or 2% GLN-supplemented TPN (GLN-TPN). In experiment 1, animals were fed chow, IV-TPN, IG-TPN, or CED for 5 days and received intraperitoneal LPS (100 microg/kg BW), and then were sacrificed 1 h later. Intestine was harvested for GALT lamina propria. Total RNA was extracted from lamina propria cells and cytokine mRNA for IL-4, and IL-10 was measured by reverse transcriptase polymerase chain reaction. IgA levels of intestinal washing were also measured with ELISA. In experiment 2, mRNA for IL-4 and IL-10, and intestinal IgA levels were measured in mice fed chow, IV-TPN, or GLN-TPN as in experiment 1. Both IL-4 and IL-10 mRNA expression decreased significantly in IV-TPN mice compared to chow or CED feeding. IG-TPN resulted in IL-10 mRNA expression significantly lower than chow or CED but significantly better than IV-TPN. GLN preserved IL-4 and IL-10 mRNA levels, which correlated with intestinal IgA levels. Route and type of nutrition as well as GLN influence message for the Th2 type IgA-stimulating cytokines, IL-4 and IL-10, within the primary site of GALT IgA production, the lamina propria. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|