In vivo reduction of reperfusion injury to the heart with apelin-12 peptide in rats
| Autor: | Maria Sidorova, Oleg I. Pisarenko, Zh. D. Bespalova, Pal'keeva Me, Irina Studneva, Yu. A. Pelogeykina, Denis N. Khatri, A. A. Az’muko, Tskitishvili Ov, L. I. Serebryakova |
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| Rok vydání: | 2012 |
| Předmět: |
Male
medicine.medical_specialty Necrosis Cardiotonic Agents Heart Ventricles Ischemia Infarction Blood Pressure Myocardial Reperfusion Injury Pharmacology General Biochemistry Genetics and Molecular Biology chemistry.chemical_compound In vivo Internal medicine Lactate dehydrogenase medicine Animals Creatine Kinase MB Form Myocardial infarction Rats Wistar Nitrites Nitrates L-Lactate Dehydrogenase General Medicine medicine.disease Apelin Rats chemistry Cardiology Intercellular Signaling Peptides and Proteins medicine.symptom Reperfusion injury |
| Zdroj: | Bulletin of experimental biology and medicine. 152(1) |
| ISSN: | 1573-8221 |
| Popis: | Apelin-12 (A-12) peptide was synthesized by automated solid phase method and purified by reverse phase HPLC. Its homogeneity and structure were confirmed by HPLC, (1)H-NMR spectroscopy, and mass spectroscopy. Acute myocardial infarction was induced by 40-min occlusion of the left coronary artery with subsequent 60-min reperfusion in narcotized Wistar rats. Peptide A-12 was injected (intravenous bolus, 0.07 or 0.35 μmol/kg) to experimental animals simultaneously with the beginning of reperfusion. Injections of A-12 in these doses led to reduction of systolic BP to 67 and 85% of the initial level, respectively, which was virtually restored completely by the end of reperfusion, and to a significant reduction of the infarction focus in the myocardium (by 21 and 34% in comparison with the control, respectively). Injection of A-12 in a dose of 0.35 μmol/kg led to reduction of plasma concentrations of necrosis markers in comparison with the control by the end of reperfusion: MB-creatine kinase by 56%, lactate dehydrogenase by 30%. The results attest to vasodilatory effects of A-12 under conditions of heart reperfusion in vivo; the peptide injected after local ischemia limits the myocardial infarction size and reduces damage to cardiomyocyte membrane. |
| Databáze: | OpenAIRE |
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