Liraglutide ameliorates COCl2-induced oxidative stress and apoptosis in H9C2 cells via regulating cell autophagy
| Autor: | Yawen Li, Lin Wang, Tao Wang, Shijun Li, Zhanqi Pang, Jian Yang, He Dong |
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| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
autophagy Cancer Research Cell medicine.disease_cause 03 medical and health sciences 0302 clinical medicine Immunology and Microbiology (miscellaneous) medicine oxidative stress Cytotoxicity chemistry.chemical_classification liraglutide Reactive oxygen species Liraglutide Autophagy apoptosis Articles General Medicine Cell cycle Cell biology 030104 developmental biology medicine.anatomical_structure chemistry Apoptosis 030220 oncology & carcinogenesis cardiovascular system Oxidative stress medicine.drug |
| Zdroj: | Experimental and Therapeutic Medicine |
| ISSN: | 1792-1015 1792-0981 |
| Popis: | Protective effects of liraglutide on H9C2 cells cultured using CoCl2 and its mechanism of action were investigated. Hypoxia model was established using CoCl2-treated H9C2 cells. With liraglutide as the treatment factor, apoptosis, changes in nitric oxide (NO) and reactive oxygen species (ROS) activity, mitochondrial membrane potential and change in cell autophagy level were detected via Hoechst staining, enzyme-linked immunosorbent assay (ELISA), polymerase chain reaction (PCR) and western blotting (WB), respectively. Liraglutide ameliorated the CoCl2-induced decrease in H9C2 cell viability, the increases in cytotoxicity and percentage of apoptotic cells as well as oxidative stress in cells. Moreover, it stimulated the elevation of cell autophagy level. However, the protective effects of liraglutide on H9C2 cells were attenuated remarkably after adding the cell autophagy inhibitor. Liraglutide can ameliorate the CoCl2-induced oxidative stress and apoptosis in H9C2 cells via regulating cell autophagy. |
| Databáze: | OpenAIRE |
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