Improvement of Virus Safety of a S/D-Treated Factor VIII Concentrate by Additional Dry Heat Treatment at 100°C
| Autor: | R. Kotitschke, H. Dichtelmüller, M. Kloft, B. Flehmig, E. Watson, G. Frösner, B. Breuer, D. Rudnick, S. Lawson, A. Darling |
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| Rok vydání: | 1996 |
| Předmět: |
Hot Temperature
viruses Bioengineering Biology medicine.disease_cause Applied Microbiology and Biotechnology Virus Microbiology Viral envelope medicine Animals Humans Pharmacology Blood Specimen Collection Factor VIII General Immunology and Microbiology Transmission (medicine) Rabies virus Hepatitis A RNA General Medicine medicine.disease biology.organism_classification Virology Solutions Kinetics Vesicular stomatitis virus Dry heat Cattle Biotechnology |
| Zdroj: | Biologicals. 24:125-130 |
| ISSN: | 1045-1056 |
| Popis: | In order to increase the virus safety of a solvent/detergent-treated Factor VIII concentrate in regard to non-lipid coated viruses and to respond to the continuous discussion about reports on hepatitis A transmission by Factor VIII preparations, we have investigated the effect of a terminal dry heat treatment (30 min 100 degrees C) on HAV and various other viruses. By this treatment Hepatitis A virus was inactivated below detectable level after a few minutes (> 5.3 log10). Other RNA viruses such as the Human Immunodeficiency Virus (> 6.6 log10), bovine viral diarrhoea virus (> 6.6 log10) and vesicular stomatitis virus (> 5.8 log10) were also inactivated below detectable level. Pseudo rabies virus and reovirus Type 3 are inactivated by 5.7 and > 6.0 log10, respectively. SV40 and bovine parvo virus showed significant resistance to dry heat treatment. We conclude that the involvement of two strong virus inactivation steps, acting by different mechanisms, improves the virus safety of Factor VIII concentrates without destroying the Factor VIII activity. Moreover, the terminal 100 degrees C heat treatment for 30 min represents an effective measure to inactivate non-lipid enveloped viruses, in particular hepatitis A, which is resistant to solvent/detergent treatment. |
| Databáze: | OpenAIRE |
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