The skin-reactive antigens of Mycoplasma pneumoniae
| Autor: | Hiromichi Mizutani, Hiroko Mizutani |
|---|---|
| Rok vydání: | 1975 |
| Předmět: |
Adult
Male Mycoplasma pneumoniae Adolescent Injections Intradermal Guinea Pigs Biology medicine.disease_cause Cell Fractionation Epitope Microbiology chemistry.chemical_compound Epitopes Sonication Mycoplasma Antigen Bacterial Proteins medicine Acetone Animals Humans Hypersensitivity Delayed Mycoplasma Infections Intradermal injection Horses Child Aged Skin Tests Antigens Bacterial integumentary system Complement Fixation Tests Age Factors General Medicine Titer chemistry Child Preschool Cattle Cell fractionation |
| Zdroj: | Japanese journal of microbiology. 19(2) |
| ISSN: | 0021-5139 |
| Popis: | Guinea pigs experimentally infected with Mycoplasma pneumoniae or immunized with the orgnaism in combination with Freund's complete adjuvant developed a delayed hypersensitive skin reaction following on intradermal injection of the M. pneumoniae antigen. The amount of protein necessary to produce the delayed skin reaction was as low as 0.01 mug. When the sonicated whole cells were extracted with aqueous acetone, the delayed skin reactivity was found mostly in the acetone insoluble (lipid-depleted) fraction. On the other hand, the lipid fraction which was isolated by a chloroform-methanol extraction of the acetone-soluble fraction and had a high titer of complement-fixing activity, exhibited little delayed skin reactivity. The lipid-depleted antigens as the whole cell antigens produced delayed skin reactivities in human patients. |
| Databáze: | OpenAIRE |
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