High synergistic antibacterial, antibiofilm, antidiabetic and antimetabolic activity of Withania somnifera leaf extract-assisted zinc oxide nanoparticle
| Autor: | Balasubramanian Malaikozhundan, Viswanathan Vinotha, Ammasi Mariyappan, Mohamed Ali Rajamohamed Kalanjiam, Subramanian Palanisamy, Jayaraj Vinodhini, Baskaralingam Vaseeharan, Sekar Vijayakumar |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Insecticides Metal Nanoparticles Nanoparticle chemistry.chemical_element Bioengineering Zinc Withania Bacterial Physiological Phenomena 01 natural sciences 010608 biotechnology Zeta potential Hypoglycemic Agents Food science Metabolic disease chemistry.chemical_classification Withania somnifera leaf extract Bacteria Plant Extracts 010405 organic chemistry Chemistry Biofilm General Medicine Antimicrobial Anti-Bacterial Agents 0104 chemical sciences Plant Leaves Enzyme Biofilms Zinc Oxide Biotechnology |
| Zdroj: | Bioprocess and Biosystems Engineering. 43:1533-1547 |
| ISSN: | 1615-7605 1615-7591 |
| Popis: | Nanotechnology is currently gaining immense attention to combat food borne bacteria, and biofilm. Diabetes is a common metabolic disease affecting majority of people. A better therapy relies on phytomediated nanoparticle synthesis. In this study, W. somnifera leaf extract-assisted ZnO NPs (Ws-ZnO NPs) was synthesized and characterized. From HR-TEM analysis, it has been found that the hexagonal wurtzite particle is 15.6 nm in size and − 12.14 mV of zeta potential. A greater antibacterial effect of Ws-ZnO NPs was noticed against E. faecalis and S. aureus at 100 µg mL−1. Also, the biofilm of E. faecalis and S. aureus was greatly inhibited at 100 µg mL−1 compared to E. coli and P. aeruginosa. The activity of α-amylase and α-glucosidase enzyme was inhibited at 100 µg mL−1 demonstrating its antidiabetic potential. The larval and pupal development was delayed at 25 µg mL−1 of Ws-ZnO NPs. A complete mortality (100%) was recorded at 25 µg mL−1. Ws-ZnO NPs showed least LC50 value (9.65 µg mL−1) compared to the uncoated ZnO NPs (38.8 µg mL−1) and leaf extract (13.06 µg mL−1). Therefore, it is concluded that Ws-ZnO NPs are promising to be used as effective antimicrobials, antidiabetic and insecticides to combat storage pests. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink