Liposomes targeted by fusion phage proteins
| Autor: | P.K. Jayanna, Petrenko Va, Vladimir P. Torchilin |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2008 |
| Předmět: |
Streptavidin
viruses Blotting Western Biomedical Engineering Pharmaceutical Science Medicine (miscellaneous) Bioengineering Peptide Biology Models Biological Article law.invention chemistry.chemical_compound law General Materials Science Bacteriophages Phage major coat protein chemistry.chemical_classification Liposome Transfection chemistry Biochemistry Membrane protein Liposomes Recombinant DNA Molecular Medicine Capsid Proteins Nanocarriers Viral Fusion Proteins |
| Popis: | Targeting of nanocarriers has long been sought after to improve the therapeutic indices of anticancer drugs. Here we provide the proof of principle for a novel approach of nanocarrier targeting through their fusion with target-specific phage coat proteins. The source of the targeted phage coat proteins are landscape phage libraries--collections of recombinant filamentous phages with foreign random peptides fused to all 4000 copies of the major coat protein. We exploit in our approach the intrinsic physicochemical properties of the phage major coat protein as a typical membrane protein. Landscape phage peptides specific for specific tumors can be obtained by affinity selection, and purified fusion coat proteins can be assimilated into liposomes to obtain specific drug-loaded nanocarriers. As a paradigm for inceptive experiments, a streptavidin-specific phage peptide selected from a landscape phage library was incorporated into approximately 100-nm liposomes. Targeting of liposomes was proved by their specific binding to streptavidin-coated beads. |
| Databáze: | OpenAIRE |
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