Non-synaptic Cholinergic Modulation of Neurogenic Twitches of the Guinea-pig Ileum
| Autor: | Karel Mašek, S. Hynie, I. Šeferna, O. Kadlec, J. S̆evc̆ík |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Atropine
Male medicine.medical_specialty Guinea Pigs Action Potentials Pharmaceutical Science In Vitro Techniques Biology Ileum Parasympathetic Nervous System Internal medicine Muscarinic acetylcholine receptor medicine Oxotremorine Animals Ouabain Pharmacology Pilocarpine Muscle Smooth Pirenzepine Acetylcholine Endocrinology Synapses Potassium Cholinergic Calcium medicine.symptom Adenylyl Cyclases Muscle Contraction medicine.drug Muscle contraction |
| Zdroj: | Journal of Pharmacy and Pharmacology. 43:342-348 |
| ISSN: | 2042-7158 0022-3573 |
| DOI: | 10.1111/j.2042-7158.1991.tb06701.x |
| Popis: | The effect of cholinergic and anticholinergic compounds on conduction of neuronal excitation has been studied in myenteric plexus-longitudinal muscle strips from the guinea-pig ileum. A preparation in a special triple bath was drawn through two rubber membranes dividing the strip into three segments. Neurogenic stimulation of the oral segment set up nerve action potentials propagating aborally across the middle segment (10 mm) so that the aboral segment might be also invaded, eventually. Drugs were added to the middle segment to affect neuronal propagation (non-synaptic effects) which was monitored by twitch height of the aboral segment. The application of acetylcholine to the middle segment augmented aboral twitches. The effects of nicotine, pilocarpine and oxotremorine were selectively blocked by (+)-tubocurarine, pirenzepine and atropine, respectively. The effect of acetylcholine was suppressed by pirenzepine and atropine and mimicked by doubling of KC1 concentration. The effect of acetylcholine may be thus explained by the facilitated propagation of nerve action potentials in partially depolarized cholinergic terminals via stimulation of muscarinic receptors. The adenylate cyclase system is not directly involved in the mechanism of muscarinic facilitation of neuronal propagation in the terminals; however, it may participate in the modulation of a final common effector mechanism. |
| Databáze: | OpenAIRE |
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