Erratum to 'Complete genome comparison of porcine reproductive and respiratory syndrome virus parental and attenuated strains'
| Autor: | Michael P. Murtaugh, Daniel J. Mickelson, Kay S. Faaberg, Shishan Yuan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2001 |
| Předmět: |
Cancer Research
Swine DNA Mutational Analysis Molecular Sequence Data Cell Culture Techniques Genome Viral Vaccines Attenuated Virus Article Open Reading Frames Species Specificity Virology Animals Porcine respiratory and reproductive syndrome virus Serial Passage Genetics Viral Structural Proteins Genome comparison biology Base Sequence Reverse Transcriptase Polymerase Chain Reaction Porcine reproductive and respiratory syndrome virus (PRRSV) Viral Vaccines Porcine reproductive and respiratory syndrome virus biology.organism_classification Infectious Diseases Amino Acid Substitution Mutation RNA Viral 5' Untranslated Regions Sequence Analysis |
| Zdroj: | Virus Research |
| ISSN: | 1872-7492 0168-1702 |
| Popis: | Two full-length porcine reproductive and respiratory syndrome virus (PRRSV) genomes, strain VR-2332 and its cell culture passaged descendent RespPRRS vaccine strain, were compared and analyzed in order to identify possible sites of attenuation. Of the 44 nucleotide changes, 13 resulted in conservative changes and 18 produced non-conservative changes. The results suggest that key amino acids in ORF1 may contribute to the phenotype of RespPRRS, which includes increased growth rate on MA-104 cells and decreased virulence in swine. The results provide a genetic basis for future manipulation of a PRRSV reverse genetics system. |
| Databáze: | OpenAIRE |
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