Media composition influences yeast one- and two-hybrid results
| Autor: | Sarah E. Bondos, Zabeena Merchant, Hao-Ching Hsiao, Kim L. Gonzalez, Ying Liu, Kathleen S. Matthews |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
false positive
yeast two-hybrid Short Report Biology computer.software_genre General Biochemistry Genetics and Molecular Biology protein-protein interaction 03 medical and health sciences False positive paradox lcsh:QH301-705.5 030304 developmental biology Yeast one-hybrid lcsh:R5-920 0303 health sciences accuracy Biochemistry Genetics and Molecular Biology(all) 030302 biochemistry & molecular biology Data interpretation false negative Replicate Composition (combinatorics) Yeast lcsh:Biology (General) Data mining lcsh:Medicine (General) Biological system computer |
| Zdroj: | Biological Procedures Biological Procedures Online, Vol 13, Iss 1, p 6 (2011) |
| ISSN: | 1480-9222 |
| DOI: | 10.1186/1480-9222-13-6 |
| Popis: | Although yeast two-hybrid experiments are commonly used to identify protein interactions, the frequent occurrence of false negatives and false positives hampers data interpretation. Using both yeast one-hybrid and two-hybrid experiments, we have identified potential sources of these problems: the media preparation protocol and the source of the yeast nitrogen base may not only impact signal range but also effect whether a result appears positive or negative. While altering media preparation may optimize signal differences for individual experiments, media preparation must be reported in detail to replicate studies and accurately compare results from different experiments. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|