Evaluation of the effectiveness of BACTEC MGIT 960 for the detection of mycobacteria in Bangladesh
Autor: | Farjana Rahman, Rashed Noor, Mst. Sabiha Banu Momi, Mehedi Hasan, Saurab Kishore Munshi |
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Rok vydání: | 2013 |
Předmět: |
Microbiology (medical)
Tuberculosis biology business.industry Tb control BACTEC MGIT 960 lcsh:QR1-502 Mycobacteria bacterial infections and mycoses biology.organism_classification medicine.disease lcsh:Microbiology Highly sensitive Microbiology Mycobacterium tuberculosis Infectious Diseases Mycobacterium tuberculosis complex Recovery rate Light emitting diode (LED) fluorescence microscopy medicine Mycobacterium tuberculosis complex (MTM) Lowenstein–Jensen culture business Non-tubercular mycobacteria (NTM) |
Zdroj: | International Journal of Mycobacteriology, Vol 2, Iss 4, Pp 214-219 (2013) |
ISSN: | 2212-5531 |
DOI: | 10.1016/j.ijmyco.2013.09.001 |
Popis: | Objective Tuberculosis (TB) caused by Mycobacterium tuberculosis has been identified as a re-emerging infectious disease with public health importance globally. Exploitation of new laboratory techniques for precise identification of mycobacteria in clinical specimens is of great importance to improve the diagnosis as part of the global TB control efforts. Methods The current study was conducted for the evaluation of BACTEC MGIT 960 method in comparison with Lowenstein–Jensen (LJ) culture and light emitting diode (LED) fluorescence microscopy for isolation of mycobacteria among TB suspects from Bangladesh. A total of 421 specimens were tested with these methods. Results Among the tested samples, 3.6% (n = 15) were LED fluorescence microscopy positive; while 18 (4.2%) and 45 (10.6%) were recovered from LJ and MGIT 960 culture. The relative positivity found through MGIT 960 system were 60% and 66.7% higher than that of LJ culture and LED fluorescence microscopy, respectively. Recovery rate of Mycobacterium tuberculosis complex ([MTC], 21 by MGIT and 16 by LJ culture) and non-tubercular mycobacteria ([NTM], 24 by MGIT and 2 by LJ culture) by MGIT 960 was 24% and 96% greater, respectively than LJ culture. Moreover, MGIT 960 was found to be highly sensitive (100%), specific (93.3%), accurate (93.6%) and a more rapid method in detecting mycobacteria when compared with LJ culture. Conclusion Extended recovery of NTM and MTC through MGIT 960 urged frequent application of this method to detect mycobacteria more effectively and rapidly. |
Databáze: | OpenAIRE |
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