In vivo evaluation of percutaneous carbon dioxide treatment for improving intratumoral hypoxia using 18F-fluoromisonidazole PET-CT
| Autor: | Eisuke Ueshima, Kayako Isohashi, Koji Sasaki, Takuya Okada, Koji Maruyama, Masato Yamaguchi, Munenobu Nogami, Takeshi Ueha, Yasukazu Kanai, Hayato Ikeda, Jun Hatazawa, Tomoyuki Gentsu, Takamichi Murakami, Keitaro Sofue, Koji Sugimoto, Yoshitada Sakai |
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| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Cancer Research 18F-Fluoromisonidazole Percutaneous intratumoral hypoxia Standardized uptake value 03 medical and health sciences 0302 clinical medicine In vivo 18F-fluoromisonidazole positron emission tomography-computed tomography Medicine PET-CT business.industry Therapeutic effect carbon dioxide Articles Hypoxia (medical) LM8 tumor in vivo 030104 developmental biology Oncology 030220 oncology & carcinogenesis Room air distribution medicine.symptom Nuclear medicine business |
| Zdroj: | Oncology Letters |
| ISSN: | 1792-1082 1792-1074 |
| Popis: | Carbon dioxide (CO2) treatment is reported to have an antitumor effect owing to the improvement in intratumoral hypoxia. Previous studies were based on histological analysis alone. In the present study, the improvement in intratumoral hypoxia by percutaneous CO2 treatment in vivo was determined using 18F-fluoromisonidazole positron emission tomography-computed tomography (18F-FMISO PET-CT) images. Twelve Japanese nude mice underwent implantation of LM8 tumor cells in the dorsal subcutaneous area 2 weeks before percutaneous CO2 treatment and 18F-FMISO PET-CT scans. Immediately after intravenous injection of 18F-FMISO, CO2 and room air were administered transcutaneously in the CO2-treated group (n=6) and a control group (n=6), respectively; each treatment was performed for 10 minutes. PET-CT was performed 2 h after administration of 18F-FMISO. 18F-FMISO tumor uptake was quantitatively evaluated using the maximum standardized uptake value (SUVmax), tumor-to-liver ratio (TLR), tumor-to-muscle ratio (TMR), metabolic tumor volume (MTV) and total lesion glycolysis (TLG). Mean ± standard error of the mean (SEM) of the tumor volume was not significantly different between the two groups (CO2-treated group, 1.178±0.450 cm3; control group, 1.368±0.295 cm3; P=0.485). Mean ± SEM of SUVmax, TLR, MTV (cm3) and TLG were significantly lower in the CO2-treated group compared with the control group (0.880±0.095 vs. 1.253±0.071, P=0.015; 1.063±0.147361 vs. 1.455±0.078, P=0.041; 0.353±0.139 vs. 1.569±0.438, P=0.015; 0.182±0.070 vs. 1.028±0.338, P=0.015), respectively. TMR was not significantly different between the two groups (4.520±0.503 vs. 5.504±0.310; P=0.240). In conclusion, 18F-FMISO PET revealed that percutaneous CO2 treatment improved intratumoral hypoxia in vivo. This technique enables assessment of the therapeutic effect in CO2 treatment by imaging, and may contribute to its clinical application. |
| Databáze: | OpenAIRE |
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