Inkjet-printed gold nanoparticle electrochemical arrays on plastic. Application to immunodetection of a cancer biomarker protein
| Autor: | Colleen E. Krause, Gary C. Jensen, James F. Rusling, Gregory A. Sotzing |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Materials science
Hot Temperature Polymers General Physics and Astronomy Nanoparticle Metal Nanoparticles Nanotechnology Substrate (printing) Article Limit of Detection Benzene Derivatives Biomarkers Tumor Electrochemistry Microelectronics Animals Humans Physical and Theoretical Chemistry Detection limit Immunoassay business.industry Interleukin-6 Amperometry Kapton Printing Cattle Ink Gold business Biosensor Plastics Polyimide |
| Popis: | Electrochemical detection combined with nanostructured sensor surfaces offers potentially low-cost, high-throughput solutions for detection of clinically significant proteins. Inkjet printing offers an inexpensive non-contact fabrication method for microelectronics that is easily adapted for incorporating into protein immunosensor devices. Herein we report the first direct fabrication of inkjet-printed gold nanoparticle arrays, and apply them to electrochemical detection of the cancer biomarker interleukin-6 (IL-6) in serum. The gold nanoparticle ink was printed on a flexible, heat resistant polyimide Kapton substrate and subsequently sintered to create eight-electrode arrays costing0.2 euro per array. The inkjet-printed working electrodes had reproducible surface areas with RSD3%. Capture antibodies for IL-6 were linked onto the eight-electrode array, and used in sandwich immunoassays. A biotinylated secondary antibody with 16-18 horseradish peroxidase labels was used, and detection was achieved by hydroquinone-mediated amperometry. The arrays provided a clinically relevant detection limit of 20 pg mL(-1) in calf serum, sensitivity of 11.4 nA pg(-1) cm(-2), and a linear dynamic range of 20-400 pg mL(-1). |
| Databáze: | OpenAIRE |
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