Practical detection of t(14;18)(IgH/BCL2) in follicular lymphoma
| Autor: | Keni Gu, Wing C. Chan, Robert C. Hawley |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Chromosomes
Human Pair 14 Paraffin Embedding Tissue Fixation General Medicine Polymerase Chain Reaction Translocation Genetic Pathology and Forensic Medicine Medical Laboratory Technology Blotting Southern Cytogenetics Genetic Techniques Humans Chromosomes Human Pair 18 Lymphoma Follicular In Situ Hybridization Fluorescence |
| Zdroj: | Archives of pathologylaboratory medicine. 132(8) |
| ISSN: | 1543-2165 |
| Popis: | The t(14;18)(q32;q21) translocation is the genetic hallmark of follicular lymphoma. Detection of this translocation can facilitate the diagnosis of follicular lymphoma and can be used to monitor response to therapy and level of residual disease. We herein review and compare practical techniques for detecting t(14;18)(q32;q21), including conventional cytogenetics, fluorescence in situ hybridization, Southern blot analysis, and polymerase chain reaction–based assay. Emphasis is placed on fluorescence in situ hybridization and polymerase chain reaction–based assay, given the applicability of these techniques to fixed, paraffin-embedded tissue. |
| Databáze: | OpenAIRE |
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