Analysis of protein adsorption characteristics to nano-pore silica particles by using confocal laser scanning microscopy
| Autor: | Jaebum Choo, Chang Woo Suh, Jong Kil Kim, Min Young Kim, Ho Kun Kim, Eun Kyu Lee |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Macromolecular Substances
Silicon dioxide Analytical chemistry Biocompatible Materials Bioengineering Applied Microbiology and Biotechnology law.invention Diffusion chemistry.chemical_compound Adsorption Confocal microscopy law Materials Testing Microscopy Particle Size Equilibrium constant Chromatography Microscopy Confocal Nanotubes Chemistry Serum Albumin Bovine General Medicine Hydrogen-Ion Concentration Silicon Dioxide Dissociation constant Particle size Porosity Protein Binding Biotechnology Protein adsorption |
| Zdroj: | Journal of Biotechnology. 112:267-277 |
| ISSN: | 0168-1656 |
| DOI: | 10.1016/j.jbiotec.2004.05.005 |
| Popis: | The effect of average pore size of nano-pore silica particles on protein adsorption characteristics was determined experimentally by the dissociation constant and the adsorption capacity determined from the Langmuir equation. As the average pore size was increased from 2.2 to 45 nm, the BSA adsorption capacity increased from 16.8 to 84.3 mg/g-silica so as the equilibrium constant (from 2.6 to 9.4 mg/ml). Using confocal microscopy with fluorescence labeling, we could visualize the protein adsorption in situ and determine the minimum pore size required for efficient intraparticle adsorption. The confocal microscopy analysis revealed that BSA was adsorbed mainly on the surface of the particles with a smaller pore size, but diffused further into the interstitial surface when it was sufficiently large. It was concluded that for BSA whose Stoke's diameter is ca. 3.55 nm the minimum pore size of about 45 nm or larger was required for a sufficient adsorption capacity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect