Modulation of behavioral and neurochemical responses of adult zebrafish by fluoxetine, eicosapentaenoic acid and lipopolysaccharide in the prolonged chronic unpredictable stress model

Autor: Allan V. Kalueff, Tatyana Strekalova, Yulia V Cherneyko, Elena Petersen, Mikael S. Mor, Tatiana O. Kolesnikova, Evgeniya V. Efimova, Dmitry V Sorokin, Nikita P. Ilyin, Yuriy M Kositsyn, Konstantin A. Demin, Konstantin N. Zabegalov, Nataliya A. Krotova, Maria V. Seredinskaya, David S. Galstyan, Nataliia A. Levchenko, Murilo S. de Abreu, Ksenia A. Derzhavina
Přispěvatelé: RS: MHeNs - R3 - Neuroscience, Psychiatrie & Neuropsychologie
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Lipopolysaccharides
0301 basic medicine
ZEBRA FISH
BLOOD
EICOSAPENTAENOIC ACID
Emotions
LIPOPOLYSACCHARIDE
Pharmacology
PHENOTYPE
FLUOXETINE
Norepinephrine
DOUBLE-BLIND
DOPAMINE
0302 clinical medicine
CHEMISTRY
ANXIETY
STRESS
PHYSIOLOGICAL
Chronic stress
PHYSIOLOGICAL STRESS
Zebrafish
Multidisciplinary
Behavior
Animal
NORADRENALIN
biology
DISEASE MODELS
ANIMAL
NEUROSCIENCE RESEARCH
Neurochemistry
Antidepressive Agents
NEUROTRANSMITTER SYSTEMS
PROINFLAMMATORY CYTOKINES
ANTIDEPRESSANT AGENT
Phenotype
EMOTIONS
Eicosapentaenoic Acid
NOREPINEPHRINE
DEPRESSION-LIKE BEHAVIOR
Medicine
MENTAL STRESS
Stress and resilience
medicine.drug
Science
METABOLISM
NEUROCHEMISTRY
BEHAVIOR
ANIMAL
Neuroprotection
Article
DISEASE MODEL
03 medical and health sciences
Neurochemical
ICOSAPENTAENOIC ACID
Stress
Physiological
Dopamine
Fluoxetine
EMOTION
medicine
Animals
Emotion
ZEBRAFISH
business.industry
LIPOPOLYSACCHARIDES
ANIMALS
ANIMAL
DOCOSAHEXAENOIC ACID
ANIMAL BEHAVIOR
biology.organism_classification
ANTIDEPRESSIVE AGENTS
Endotoxins
Disease Models
Animal
CHRONIC MILD STRESS
030104 developmental biology
DRUG THERAPY
PROCEDURES
ENDOTOXINS
ENDOTOXIN
PITUITARY-ADRENAL AXIS
STRESS
PSYCHOLOGICAL
business
Stress
Psychological
030217 neurology & neurosurgery
Neuroscience
Zdroj: Scientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
Sci. Rep.
Scientific Reports
Scientific Reports, 11(1):14289. Nature Publishing Group
ISSN: 2045-2322
Popis: Long-term recurrent stress is a common cause of neuropsychiatric disorders. Animal models are widely used to study the pathogenesis of stress-related psychiatric disorders. The zebrafish (Danio rerio) is emerging as a powerful tool to study chronic stress and its mechanisms. Here, we developed a prolonged 11-week chronic unpredictable stress (PCUS) model in zebrafish to more fully mimic chronic stress in human populations. We also examined behavioral and neurochemical alterations in zebrafish, and attempted to modulate these states by 3-week treatment with an antidepressant fluoxetine, a neuroprotective omega-3 polyunsaturated fatty acid eicosapentaenoic acid (EPA), a pro-inflammatory endotoxin lipopolysaccharide (LPS), and their combinations. Overall, PCUS induced severe anxiety and elevated norepinephrine levels, whereas fluoxetine (alone or combined with other agents) corrected most of these behavioral deficits. While EPA and LPS alone had little effects on the zebrafish PCUS-induced anxiety behavior, both fluoxetine (alone or in combination) and EPA restored norepinephrine levels, whereas LPS + EPA increased dopamine levels. As these data support the validity of PCUS as an effective tool to study stress-related pathologies in zebrafish, further research is needed into the ability of various conventional and novel treatments to modulate behavioral and neurochemical biomarkers of chronic stress in this model organism. © 2021, The Author(s). This research was supported solely by the Russian Science Foundation (RSF) grant 19‐15‐00053. K.A.D. is supported by the Special Rector’s Productivity Fellowship for SPSU PhD Students, and the lab is supported by St. Petersburg State University state budgetary funds (project ID 73026081). A.V.K. is the Chair of the International Zebrafish Neuroscience Research Consortium (ZNRC) and President of the International Stress and Behavior Society (ISBS, www.stress-and-behavior.com) that coordinated this collaborative multi-laboratory project. The consortium provided a collaborative idea exchange platform for this study, it is not considered as affiliation and did not fund the study. A.V.K. lab is supported by the Southwest University (SWU) Zebrafish Platform Construction Fund (Chongqing, China). The authors thank Professor Raul R. Gainetdinov (Institute of Translational Biomedicine, St. Petersburg State University, St. Petersburg, Russia) for his generous assistance with the HPLC studies in his laboratory. The funders had no role in the design, analyses, and interpretation of the submitted study, or decision to publish.
Databáze: OpenAIRE
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