Adipose-Derived Stem Cells Promote Peripheral Nerve Regeneration In Vivo without Differentiation into Schwann-Like Lineage
| Autor: | Yasuhiko Tabata, Toshiaki Numajiri, Osam Mazda, Tetsuya Imura, Yoshihiro Sowa, Tsunao Kishida, Kenichi Nishino |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Nerve guidance conduit Schwann cell Clinical uses of mesenchymal stem cells Mice 03 medical and health sciences 0302 clinical medicine Peripheral Nerve Injuries Adipocytes Animals Medicine Peripheral Nerves Stem cell transplantation for articular cartilage repair business.industry Cell Differentiation Amniotic stem cells Nerve Regeneration 030104 developmental biology medicine.anatomical_structure nervous system Peripheral nerve injury Surgery Schwann Cells Stem cell business 030217 neurology & neurosurgery Stem Cell Transplantation Adult stem cell |
| Zdroj: | Plastic and Reconstructive Surgery. 137:318e-330e |
| ISSN: | 0032-1052 |
| DOI: | 10.1097/01.prs.0000475762.86580.36 |
| Popis: | Background During recent decades, multipotent stem cells were found to reside in the adipose tissue, and these adipose-derived stem cells were shown to play beneficial roles, like those of Schwann cells, in peripheral nerve regeneration. However, it has not been well established whether adipose-derived stem cells offer beneficial effects to peripheral nerve injuries in vivo as Schwann cells do. Furthermore, the in situ survival and differentiation of adipose-derived stem cells after transplantation at the injured peripheral nerve tissue remain to be fully elucidated. Methods Adipose-derived stem cells and Schwann cells were transplanted with gelatin hydrogel tubes at the artificially blunted sciatic nerve lesion in mice. Neuroregenerative abilities of them were comparably estimated. Cre-loxP-mediated fate tracking was performed to visualize survival in vivo of transplanted adipose-derived stem cells and to investigate whether they differentiated into Schwann linage cells at the peripheral nerve injury site. Results The transplantation of adipose-derived stem cells promoted regeneration of axons, formation of myelin, and restoration of denervation muscle atrophy to levels comparable to those achieved by Schwann cell transplantation. The adipose-derived stem cells survived for at least 4 weeks after transplantation without differentiating into Schwann cells. Conclusions Transplanted adipose-derived stem cells did not differentiate into Schwann cells but promoted peripheral nerve regeneration at the injured site. The neuroregenerative ability was comparable to that of Schwann cells. Adipose-derived stem cells at an undifferentiated stage may be used as an alternative cell source for autologous cell therapy for patients with peripheral nerve injury. |
| Databáze: | OpenAIRE |
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