Photo-induced chemistry for the design of oligonucleotide conjugates and surfaces
| Autor: | Ishtiaq Ahmed, Christof M. Niemeyer, Antonina Vigovskaya, Doris Abt, Ljiljana Fruk, Christopher Barner-Kowollik |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Diene
Chemistry Oligonucleotide Biomedical Engineering A protein 02 engineering and technology General Chemistry General Medicine 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Cycloaddition 0104 chemical sciences chemistry.chemical_compound Light induced Organic chemistry General Materials Science 0210 nano-technology DNA Conjugate |
| Zdroj: | Journal of Materials Chemistry B. 4:442-449 |
| ISSN: | 2050-7518 2050-750X |
| DOI: | 10.1039/c5tb02207j |
| Popis: | A photocaged diene is introduced at the 5'-end of oligonucleotides using the H-phosphonate approach. The photoenol-functionalized DNA is subsequently employed for the conjugation to a protein and the spatially controlled immobilization onto surfaces using a light-induced Diels-Alder cycloaddition. Fully functional protein-DNA conjugates and patterned DNA surfaces are obtained under mild irradiation conditions. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|