An ultraconserved Hox–Pbx responsive element resides in the coding sequence of Hoxa2 and is active in rhombomere 4
| Autor: | Sophie Remacle, Omar Abdel O.A. Samad, Xavier Lampe, Jacques J. Picard, Allan Guiguen, René Rezsohazy, Filippo F.M. Rijli, Christelle Matis |
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| Přispěvatelé: | Unit of Developmental Genetics, Université Catholique de Louvain = Catholic University of Louvain (UCL), Institut de génétique et biologie moléculaire et cellulaire (IGBMC), Université Louis Pasteur - Strasbourg I-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Unité de Recherche en Biologie Moléculaire, Facultés Universitaires Notre Dame de la Paix (FUNDP), Unit of Veterinary Sciences, Institut des Sciences de la Vie, Peney, Maité, UCL - SC/BIOL - Département de biologie |
| Rok vydání: | 2008 |
| Předmět: |
Transcriptional Activation
animal structures Rhombomere Hindbrain Chick Embryo Computational biology MESH: Base Sequence Biology Response Elements Cell Line Conserved sequence Mice 03 medical and health sciences 0302 clinical medicine MESH: Homeodomain Proteins Genetics Animals Coding region MESH: Animals Hox gene Enhancer Molecular Biology MESH: Mice Conserved Sequence 030304 developmental biology Homeodomain Proteins 0303 health sciences Binding Sites MESH: Conserved Sequence Base Sequence MESH: Rhombencephalon MESH: Transcription Factors MESH: Chick Embryo [SDV.ETH] Life Sciences [q-bio]/Ethics MESH: Cell Line [SDV.ETH]Life Sciences [q-bio]/Ethics 3. Good health Chromatin Rhombencephalon MESH: Binding Sites MESH: Trans-Activation (Genetics) Regulatory sequence embryonic structures MESH: Response Elements Biologie 030217 neurology & neurosurgery Transcription Factors |
| Zdroj: | Nucleic acids research, 36 (10 Nucleic Acids Research Nucleic Acids Research, 2008, 36 (10), pp.3214-25. ⟨10.1093/nar/gkn148⟩ Nucleic Acids Research, Oxford University Press, 2008, 36 (10), pp.3214-25. ⟨10.1093/nar/gkn148⟩ Nucleic Acids Research, Vol. 36, no. 10, p. 3214-25 (2008) |
| ISSN: | 1362-4962 0305-1048 |
| DOI: | 10.1093/nar/gkn148 |
| Popis: | The Hoxa2 gene has a fundamental role in vertebrate craniofacial and hindbrain patterning. Segmental control of Hoxa2 expression is crucial to its function and several studies have highlighted transcriptional regulatory elements governing its activity in distinct rhombomeres. Here, we identify a putative Hox-Pbx responsive cis-regulatory sequence, which resides in the coding sequence of Hoxa2 and is an important component of Hoxa2 regulation in rhombomere (r) 4. By using cell transfection and chromatin immunoprecipitation (ChIP) assays, we show that this regulatory sequence is responsive to paralogue group 1 and 2 Hox proteins and to their Pbx co-factors. Importantly, we also show that the Hox-Pbx element cooperates with a previously reported Hoxa2 r4 intronic enhancer and that its integrity is required to drive specific reporter gene expression in r4 upon electroporation in the chick embryo hindbrain. Thus, both intronic as well as exonic regulatory sequences are involved in Hoxa2 segmental regulation in the developing r4. Finally, we found that the Hox-Pbx exonic element is embedded in a larger 205-bp long ultraconserved genomic element (UCE) shared by all vertebrate genomes. In this respect, our data further support the idea that extreme conservation of UCE sequences may be the result of multiple superposed functional and evolutionary constraints. © 2008 The Author(s). SCOPUS: ar.j info:eu-repo/semantics/published |
| Databáze: | OpenAIRE |
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