Validation of microRNAs in Cerebrospinal Fluid as Biomarkers for Different Forms of Dementia in a Multicenter Study
| Autor: | Naomi De Roeck, Davide Chiasserini, Mareike Müller, Jill Luyckx, Jurgen A.H.R. Claassen, Hanne Struyfs, Francesca Baschieri, Marcel M. Verbeek, Lucilla Parnetti, Sebastiaan Engelborghs, Lucia Farotti, H. Bea Kuiperij, Alexandra A M Versleijen |
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| Přispěvatelé: | Clinical sciences, Neurology |
| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Oncology Pathology Alzheimer`s disease Donders Center for Medical Neuroscience [Radboudumc 1] Lewy Body Disease/cerebrospinal fluid Disease Alzheimer Disease/cerebrospinal fluid 0302 clinical medicine Cerebrospinal fluid MicroRNAs/cerebrospinal fluid Medicine Medicine(all) General Neuroscience Confounding General Medicine Alzheimer's disease Middle Aged Disorders of movement Donders Center for Medical Neuroscience [Radboudumc 3] Psychiatry and Mental health Clinical Psychology Dementia/cerebrospinal fluid Frontotemporal Dementia Disease Progression Female Frontotemporal dementia Lewy Body Disease medicine.medical_specialty cerebrospinal fluid 03 medical and health sciences Cognitive Dysfunction/cerebrospinal fluid mild cognitive impairment Alzheimer Disease Internal medicine Humans Dementia Biomarkers/cerebrospinal fluid Cognitive Dysfunction Aged business.industry Dementia with Lewy bodies Case-control study medicine.disease MicroRNAs 030104 developmental biology frontotemporal dementia Lewy body disease microRNAs Psychiatry and Mental Health Geriatrics and Gerontology Case-Control Studies Human medicine Frontotemporal Dementia/cerebrospinal fluid business Biomarkers 030217 neurology & neurosurgery |
| Zdroj: | Journal of Alzheimer's disease Journal of Alzheimer's Disease, 52, 4, pp. 1321-33 Journal of Alzheimer's Disease, 52, 1321-33 |
| ISSN: | 1875-8908 1387-2877 |
| Popis: | Item does not contain fulltext MicroRNAs (miRNAs) regulate translational inhibition of proteins, but are also detected in body fluids, including cerebrospinal fluid (CSF), where they may serve as disease-specific biomarkers. Previously, we showed differential expression of miR-146a, miR-29a, and miR-125b in the CSF of Alzheimer's disease (AD) patients versus controls. In this study, we aim to confirm these findings by using larger, independent sample cohorts of AD patients and controls from three different centers. Furthermore, we aim to identify confounding factors that possibly arise using such a multicenter approach. The study was extended by including patients diagnosed with mild cognitive impairment due to AD, frontotemporal dementia and dementia with Lewy bodies. Previous results of decreased miR-146a levels in AD patients compared to controls were confirmed in one center. When samples from all three centers were combined, several confounding factors were identified. After controlling for these factors, we did not identify differences in miRNA levels between the different groups. However, we provide suggestions to circumvent various pitfalls when measuring miRNAs in CSF to improve future studies. |
| Databáze: | OpenAIRE |
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