Kinetic and thermodynamic of lactoferrin – Ethoxylated-nonionic surfactants supramolecular complex formation
Autor: | Ana Clarissa dos Santos Pires, Eliara Acipreste Hudson, Alan S. B. de Castro, Hauster Maximiler Campos de Paula, Yara Luiza Coelho, Luis Henrique Mendes da Silva |
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Rok vydání: | 2021 |
Předmět: |
Chemistry
Entropy Activated complex Temperature Supramolecular chemistry Thermodynamics General Medicine Hydrogen-Ion Concentration Surface Plasmon Resonance Kinetic energy Biochemistry Hydrophobic effect Kinetics Lactoferrin Surface-Active Agents Adsorption Structural Biology Desorption Cetomacrogol Surface plasmon resonance Hydrophobic and Hydrophilic Interactions Molecular Biology Protein Binding Entropy (order and disorder) |
Zdroj: | International Journal of Biological Macromolecules. 187:325-331 |
ISSN: | 0141-8130 |
Popis: | Understanding nonionic surfactant-protein interactions is fundamental from both technological and scientific points of view. However, there is a complete absence of kinetic data for such interactions. We employed surface plasmon resonance (SPR) to determine the kinetic and thermodynamic parameters of bovine lactoferrin-Brij58 interactions at various temperatures under physiological conditions (pH 7.4). The adsorption process was accelerated with increasing temperature, while the desorption rate decreased, resulting in a more thermodynamically stable complex. The kinetic energetic parameters obtained for the formation of the activated complex, [bLF-Brij58]‡, indicated that the potential energy barrier for [bLF-Brij58]‡ formation arises primarily from the reduction in system entropy. [bLF-Brij58]○ formation was entropically driven, indicating that hydrophobic interactions play a fundamental role in bLF interactions with Brij58. |
Databáze: | OpenAIRE |
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