Time-lapse imaging reveals differences in growth dynamics of embryos after in vitro maturation compared with conventional stimulation
| Autor: | Jens-Erik Dietrich, Markus Montag, Thomas Strowitzki, Bettina Toth, Julia Weigert, Sabine Roesner |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Adult medicine.medical_specialty Pregnancy Rate medicine.medical_treatment Embryonic Development Biology Time-Lapse Imaging Intracytoplasmic sperm injection Embryo Culture Techniques Hospitals University 03 medical and health sciences Young Adult 0302 clinical medicine Pregnancy Internal medicine medicine Humans Sperm Injections Intracytoplasmic Retrospective Studies 030219 obstetrics & reproductive medicine Microscopy Video Pronucleus Embryogenesis Obstetrics and Gynecology Embryo medicine.disease Embryo Transfer Polycystic ovary In vitro maturation In Vitro Oocyte Maturation Techniques Kinetics Meiosis 030104 developmental biology Endocrinology Blastocyst Fertility Treatment Outcome Reproductive Medicine Infertility embryonic structures Female Live birth Live Birth Polycystic Ovary Syndrome |
| Zdroj: | Fertility and sterility. 107(3) |
| ISSN: | 1556-5653 |
| Popis: | Objective To study the impact of in vitro maturation (IVM) on embryonal development with the use of time-lapse imaging. Design Retrospective case-control study. Setting University hospital. Patient(s) In total, 294 embryos were cultured after intracytoplasmic sperm injection treatment of three groups: patients with polycystic ovary syndrome (PCOS) and IVM (n = 105; group 1 [G1]), patients after conventional stimulation without PCOS (n = 115; G2) and with PCOS (n = 74; G3). In total, 171 embryos were finally analyzed (57 G1, 65 G2, and 49 G3). Intervention(s) Data of 23 PCOS patients (30 IVM cycles) from January 2012 to July 2015 were matched according to age and number of oocytes to patients after conventional stimulation without PCOS (n = 30; 30 cycles) and with PCOS (n = 16; 19 cycles). Markers of embryo development were analyzed at different time points. Pregnancy rates (PRs) and live birth rates (LBRs) were recorded. Main Outcome Measure(s) Morphokinetic differences in embryo development after IVM compared with conventional stimulation with or without PCOS. Result(s) The rate of good-quality embryos was significantly lower in G1. Embryo development in G1 was significantly accelerated to the time of appearance of two pronuclei but slowed down by the time of reaching 6-cell stage and remained slower compared with embryos of G2 and G3. PRs as well as LBRs did not differ significantly among the study groups. Conclusion(s) Although growth dynamics of embryos from G1 differ from G2 and G3 and the rate of good-quality embryos was lower in IVM embryos, PRs and LBRs did not differ significantly. |
| Databáze: | OpenAIRE |
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