The basal release of endothelium-derived catecholamines regulates the contractions of Chelonoidis carbonaria aorta caused by electrical-field stimulation

Autor: Guilherme M. Figueiredo Murari, David Halen Araújo Pinheiro, Fabíola Z. Mónica, Edson Antunes, Gilberto De Nucci, Felipe Fernandes Jacintho, Ronilson Agnaldo Moreno, José Britto-Júnior, Valéria Barbosa de Souza, Rafael Campos, André Almeida Schenka
Rok vydání: 2021
Předmět:
medicine.medical_specialty
QH301-705.5
medicine.drug_class
Dopamine
l-name
Science
vessel
030232 urology & nephrology
Biology
030226 pharmacology & pharmacy
General Biochemistry
Genetics and Molecular Biology
Receptors
Dopamine
03 medical and health sciences
chemistry.chemical_compound
Catecholamines
0302 clinical medicine
Phentolamine
Tandem Mass Spectrometry
tyrosine hydroxylase
Internal medicine
medicine.artery
ÓXIDO NÍTRICO
medicine
Animals
lc-ms-ms
Biology (General)
Tyrosine Hydroxylase Inhibitor
Aorta
tortoise
SCH-23390
Tyrosine hydroxylase
Receptor antagonist
Immunohistochemistry
Electric Stimulation
Turtles
Endocrinology
chemistry
odq
cardiovascular system
Catecholamine
Endothelium
Vascular
General Agricultural and Biological Sciences
Research Article
Chromatography
Liquid
Muscle Contraction
medicine.drug
Zdroj: Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual)
Universidade de São Paulo (USP)
instacron:USP
Biology Open, Vol 10, Iss 1 (2021)
Biology Open
article-version (VoR) Version of Record
Popis: The contractions of Chelonoidis carbonaria aortic rings induced by electrical field stimulation (EFS) are not inhibited by blockade of the voltage-gated sodium channels by tetrodotoxin but almost abolished by the α1/α2-adrenoceptor antagonist phentolamine. The objective of this study was to identify the mediator(s) responsible for the EFS-induced contractions of Chelonoidis carbonaria aortic rings. Each ring was suspended between two wire hooks and mounted in isolated 10 ml organ chambers filled with oxygenated and heated Krebs-Henseleit's solution. Dopamine, noradrenaline and adrenaline concentrations were analysed by liquid chromatography coupled to tandem mass spectrometry. The contractions caused by dopamine and EFS were done in absence and presence of the nitric oxide (NO) synthesis inhibitor L-NAME, the NO-sensitive guanylyl cyclase inhibitor ODQ, the D1-like receptor antagonist SCH-23390, the D2-like receptor antagonists risperidone, quetiapine, haloperidol, and the tyrosine hydroxylase inhibitors salsolinol and 3-iodo-L-tyrosine. Basal concentrations of dopamine, noradrenaline and adrenaline were detected in Krebs-Henseleit solution containing the aortic rings. The catecholamine concentrations were significantly reduced in endothelium-denuded aortic rings. L-NAME and ODQ significantly potentiated the dopamine-induced contractions. The D2-like receptor antagonists inhibited the EFS-induced contractions of the aortic rings treated with L-NAME, whereas SCH 23390 had no effect. Similar results were observed in the contractions induced by dopamine in L-NAME treated aortic rings. These results indicate that catecholamines released by endothelium regulate the EFS-induced contractions. This may constitute a suitable mechanism by which reptilia modulate specific organ blood flow distribution. This paper has an associated First Person interview with the first author of the article.
Summary: Chelonoidis carbonaria aortic rings release endothelium-derived catecholamines that modulate vascular smooth muscle reactivity.
Databáze: OpenAIRE
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