Improved access to CD20 following B cell receptor cross-linking at Burkitt’s lymphoma cell surfaces
| Autor: | John Gordon, Michelle J. Holder, Anita Chamba, Debbie L. Hardie, Julie P. Deans |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Cancer Research
B-cell receptor Receptors Antigen B-Cell Biology Endocytosis medicine.disease_cause CD19 chemistry.chemical_compound immune system diseases Cell Line Tumor hemic and lymphatic diseases medicine Animals Lipid raft Cytochalasin D breakpoint cluster region Hematology Antigens CD20 medicine.disease Burkitt Lymphoma Epstein–Barr virus Cell biology Oncology chemistry biology.protein Burkitt's lymphoma |
| Zdroj: | Leukemia Research. 28:1197-1202 |
| ISSN: | 0145-2126 |
| DOI: | 10.1016/j.leukres.2004.02.008 |
| Popis: | Here we report that B cell receptor (BCR) engagement rapidly improves the capacity of CD20 to be accessed by cognate antibody at model Burkitt’s lymphoma cell surfaces. None of eight other surface molecules demonstrated such BCR-dependent enhancement of ligand binding while the quantity of accessible CD20 remained unchanged on either CD19 or CD40 engagement. Neither the actin-depolymerizing agent cytochalasin D nor inhibitors targeting signalling pathways associated with the BCR attenuated the CD20 increase that could be uncoupled from BCR endocytosis. Instead, a role for lipid rafts was indicated both from the inhibitory actions of cholesterol-sequestering methyl-β-cyclodextrin and direct analysis of CD20 redistribution using sucrose density gradients and confocal microscopy. Whether such observations could find application in CD20-directed therapies where success can be compromised by otherwise low-level expression of target antigen is discussed. |
| Databáze: | OpenAIRE |
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