5-Hydroxytryptamine-activated single-channel currents recorded from murine neuroblastoma cells
| Autor: | Peter N.R. Usherwood, F. Guharay, R. L. Ramsey |
|---|---|
| Rok vydání: | 1985 |
| Předmět: |
Serotonin
Chemistry General Neuroscience Action Potentials Conductance Depolarization Anatomy Resting potential Ion Channels Cell Line Rats Kinetics Neuroblastoma Membrane Biophysics Animals Neurology (clinical) Patch clamp Reversal potential Receptor Molecular Biology Cells Cultured 5-HT receptor Developmental Biology |
| Zdroj: | Brain Research. 340:325-332 |
| ISSN: | 0006-8993 |
| DOI: | 10.1016/0006-8993(85)90929-1 |
| Popis: | Differentiated murine neuroblastoma cells (NIE-115) have receptors for 5-hydroxytryptamine (5-HT), the activation of which either depolarizes or hyperpolarizes these cells with associated increases in input conductance. Ionophoresis of 5-HT on to NIE-115 cells elicits brief depolarization, but the 5-HT receptors rapidly desensitize during repeated application of 5-HT. The reversal potential of 5-HT-induced depolarization is ca. 0 mV. With the use of the improved patch clamp technique it is possible to record single ion channel currents activated by 5-HT from intact cells as well as isolated membrane patches. These currents are invariably inward at the resting potential (approximately 50 mV). The channel has a conductance of approximately 140 pS and reversal potential of approximately 0 mV in normal saline. The channels recorded from the intact cells reveal more than one kinetically distinguishable open and closed states. The kinetic properties of 5-HT channels recorded from excised patches of membrane (as outside-out) are different from those recorded from the intact cell. Possible explanations for such differences are presented. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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