Analysis of a Reverse Transcription Loop-mediated Isothermal Amplification (RT-LAMP) for yellow fever diagnostic
| Autor: | Robert B. Tesh, Keley N. B Nunes, Lívia Carício Martins, Sandro Patroca da Silva, Márcio Roberto Teixeira Nunes, Valéria Lima Carvalho, Hilda Guzman, Clayton Pereira Silva de Lima, Pedro Fernando da Costa Vasconcelos, João L.S.G. Vianez |
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| Rok vydání: | 2015 |
| Předmět: |
Molecular Sequence Data
Context (language use) Human pathogen Biology Viral Nonstructural Proteins Genome Virus Virology Yellow Fever medicine V?rus da Febre Amarela / isolamento & purifica??o Humans Diagnostic Reverse Transcription Loop-mediated Isothermal Amplification RT-LAMP DNA Primers Febre Amarela / virologia Base Sequence Yellow fever Outbreak Central America Nucleic acid amplification technique South America medicine.disease Genome detection T?cnicas de Amplifica??o de ?cido Nucleico / m?todos Caribbean Region Population Surveillance Africa Rea??o em Cadeia da Polimerase Via Transcriptase Reversa / m?todos Febre Amarela / diagn?stico Transcri??o Reversa Yellow fever virus Nucleic Acid Amplification Techniques |
| Zdroj: | Repositório Digital do Instituto Evandro Chagas (Patuá) Instituto Evandro Chagas (IEC) instacron:IEC |
| ISSN: | 1879-0984 |
| Popis: | This study was partially supported by CNPq (Conselho Nacional para o Desenvolvimento Cient?fico e Tecnol?gico) projects 401558/2013-4; 457664/2013-4, 486069/2012-5 and 301641/2010-2), and CNPq/CAPES/FAPESPA (project 573739-2008-0). Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Centro de Inova??es Tecnol?gicas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Centro de Inova??es Tecnol?gicas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Centro de Inova??es Tecnol?gicas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Centro de Inova??es Tecnol?gicas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Centro de Inova??es Tecnol?gicas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil. Minist?rio da Sa?de. Secretaria de Vigil?ncia em Sa?de. Instituto Evandro Chagas. Ananindeua, PA, Brasil / University of Para State. Department of Pathology. Belem, PA, Brazil. University of Texas Medical Branch. Department of Pathology. Galveston, TX, USA. University of Texas Medical Branch. Department of Pathology. Galveston, TX, USA. Yellow Fever virus (YFV) is an important human pathogen in tropical areas of Africa and South America. Although an efficient vaccine is available and has been used since the early 1940s, sylvatic YFV transmission still occurs in forested areas where anthropogenic actions are present, such as mineral extraction, rearing livestock and agriculture, and ecological tourism. In this context, two distinct techniques based on the RT-PCR derived method have been previously developed, however both methods are expensive due to the use of thermo cyclers and labeled probes. We developed isothermal genome amplification, which is a rapid, sensitive, specific and low cost molecular approach for YFV genome detection. This assay used a set of degenerate primers designed for the NS1 gene and was able to amplify, within 30 min in isothermal conditions, the YFV 17D vaccine strain derived from an African wild prototype strain (Asibi),as well as field strains from Brazil, other endemic countries from South and Central America, and the Caribbean. The generic RT-LAMP assay could be helpful for YFV surveillance in field and rapid response during outbreaks in endemic areas. |
| Databáze: | OpenAIRE |
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