Control of pore structure and size in freeze-dried collagen sponges
| Autor: | Jörn Apel, H. Schoof, Ingo Heschel, Günter Rau |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Materials science
Morphology (linguistics) biology Scanning electron microscope Biomedical Engineering Biomaterial Nanotechnology Biocompatible Materials biology.organism_classification Suspension (chemistry) Biomaterials Sponge Freeze-drying Freeze Drying Chemical engineering Tissue engineering Unidirectional solidification Collagen |
| Zdroj: | Journal of biomedical materials research. 58(4) |
| ISSN: | 0021-9304 |
| Popis: | Because of many suitable properties, collagen sponges are used as an acellular implant or a biomaterial in the field of tissue engineering. Generally, the inner three-dimensional structure of the sponges influences the behavior of cells. To investigate this influence, it is necessary to develop a process to produce sponges with a defined, adjustable, and homogeneous pore structure. Collagen sponges can be produced by freeze-drying of collagen suspensions. The pore structure of the freeze-dried sponges mirrors the ice-crystal morphology after freezing. In industrial production, the collagen suspensions are solidified under time- and space-dependent freezing conditions, resulting in an inhomogeneous pore structure. In this investigation, unidirectional solidification was applied during the freezing process to produce collagen sponges with a homogeneous pore structure. Using this technique the entire sample can be solidified under thermally constant freezing conditions. The ice-crystal morphology and size can be adjusted by varying the solute concentration in the collagen suspension. Collagen sponges with a very uniform and defined pore structure can be produced. Furthermore, the pore size can be adjusted between 20-40 microm. The thickness of the sponges prepared during this research was 10 mm. |
| Databáze: | OpenAIRE |
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