Stanozolol promotes osteogenic gene expression and apposition of bone mineral in vitro
| Autor: | Daniele Mori, G. Ghiacci, Guido Maria Macaluso, Roberto Sala, Edoardo Manfredi, Simone Lumetti |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Time Factors Bone matrix Core Binding Factor Alpha 1 Subunit Real-Time Polymerase Chain Reaction Calcitriol receptor Bone remodeling Calcification Andrology 03 medical and health sciences Anabolic Agents Calcification Physiologic Osteogenesis Cell Line Tumor Bone cell Humans Osteonectin Osteopontin General Dentistry Bone mineral Analysis of Variance Osteoblasts biology Chemistry Reproducibility of Results lcsh:RK1-715 RUNX2 Apposition 030104 developmental biology lcsh:Dentistry Linear Models biology.protein Androgens Receptors Calcitriol Original Article Gene expression Stanozolol |
| Zdroj: | Journal of Applied Oral Science, Volume: 27, Article number: e20180014, Published: 08 NOV 2018 Journal of Applied Oral Science (Impr.) 27 (2019): e20180014-1. doi:10.1590/1678-7757-2018-0014 info:cnr-pdr/source/autori:Ghiacci, Giulia; Lumetti, Simone; Manfredi, Edoardo; Mori, Daniele; Macaluso, Guido Maria; Sala, Roberto/titolo:Stanozolol promotes osteogenic gene expression and apposition of bone mineral in vitro/doi:10.1590%2F1678-7757-2018-0014/rivista:Journal of Applied Oral Science (Impr.)/anno:2019/pagina_da:e20180014-1/pagina_a:/intervallo_pagine:e20180014-1/volume:27 Journal of Applied Oral Science, Vol 27, Iss 0 (2018) Journal of Applied Oral Science Journal of Applied Oral Science v.27 2019 Journal of applied oral science Universidade de São Paulo (USP) instacron:USP |
| Popis: | Stanozolol (ST) is a synthetic androgen with high anabolic potential. Although it is known that androgens play a positive role in bone metabolism, ST action on bone cells has not been sufficiently tested to support its clinical use for bone augmentation procedures. Objective: This study aimed to assess the effects of ST on osteogenic activity and gene expression in SaOS-2 cells. Material and Methods: SaOS-2 deposition of mineralizing matrix in response to increasing doses of ST (0-1000 nM) was evaluated through Alizarin Red S and Calcein Green staining techniques at 6, 12 and 24 days. Gene expression of runt-related transcription factor 2 (RUNX2), vitamin D receptor (VDR), osteopontin (SPP1) and osteonectin (ON) was analyzed by RT-PCR. Results: ST significantly influenced SaOS-2 osteogenic activity: stainings showed the presence of rounded calcified nodules, which increased both in number and in size over time and depending on ST dose. RT-PCR highlighted ST modulation of genes related to osteogenic differentiation. Conclusions: This study provided encouraging results, showing ST promoted the osteogenic commitment of SaOS-2 cells. Further studies are required to validate these data in primary osteoblasts and to investigate ST molecular pathway of action. |
| Databáze: | OpenAIRE |
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