Expression of Beta-2-lntegrins and I-Seiectin by Leukocytes and Changes in Acute-Phase Reactants in Total Hip Replacement Surgery
| Autor: | Torstein Lyberg, H. Kähler, H.E. Høgevold, Olav Reikerås |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Adult
Male medicine.medical_specialty medicine.drug_class medicine.medical_treatment Antithrombin III Monoclonal antibody Leukocyte Count Internal medicine Leukocytes medicine Humans Postoperative Period L-Selectin skin and connective tissue diseases Beta (finance) Interleukin 6 Aged biology Interleukin-6 Cell adhesion molecule business.industry C-reactive protein Acute-phase protein Middle Aged C-Reactive Protein Endocrinology Cytokine CD18 Antigens Immunology biology.protein Female Surgery L-selectin Hip Prosthesis sense organs business Cell Adhesion Molecules Acute-Phase Proteins Granulocytes Peptide Hydrolases |
| Zdroj: | European Surgical Research. 28:190-200 |
| ISSN: | 1421-9921 0014-312X |
| DOI: | 10.1159/000129457 |
| Popis: | The aim of this study was to characterize the changes in the quantitative expression of beta 2-integrins and L-selectin detected by means of fluorochrome-conjugated monoclonal antibodies and flow cytometry on leukocytes in the systemic circulation after a major musculoskeletal trauma, i.e. hip replacement surgery, and to relate these changes to parameters of the acute-phase response [plasma acute-phase reactants (C-reactive protein, CRP, and interleukin-6, IL-6) and parameters of coagulation activation (thrombin-antithrombin III complexes, TAT)]. Eight patients with either primary or secondary osteoarthritis of the hip received uncemented total hip prostheses. LFA-1 (CD11a/CD18) was upregulated on granulocytes during the operation. MAC-1 (CD11b/CD18) expression on monocytes increased to peak levels 20 h after surgery, whereas the L-selectin (CD62L) expression on monocytes and granulocytes reached peak values at the end of surgery. The changes in expression of LFA-1 on monocytes, MAC-1 on granulocytes and p150,95 (CD11c/CD18) on monocytes and granulocytes during and after the operation did not reach statistical significance. TAT and IL-6 increased during surgery and reached peak values at the end of the operation and 20 h after surgery, respectively. In contrast, CPR concentrations increased after surgery with peak levels 44 h postoperatively. Significant upregulation of LFA-1 on granulocytes and L-selectin on monocytes and granulocytes preceded the increase in IL-6 which again preceded the increase in CRP. However, the up- or downregulation of leukocyte beta 2-integrins and L-selectin during and after surgery was not significantly correlated with the increase in IL-6. The increases in TAT correlated well with the upregulation of L-selectin on monocytes, but not with the beta 2-integrins known to participate in the coagulation process in vitro. The rise in CRP was inversely correlated with the maximal increase in expression of MAC-1 on monocytes. In conclusion, the changes in leukocyte adhesion molecules during and after surgery indicate changes in critical leukocyte functions. The lack of correlation between quantitative up- and downregulation of leukocyte beta 2-integrins and parameters of the acute phase response suggests that these processes are regulated through independent pathways or that functional up- and downregulation of adhesion molecules, shedding, leukocyte-endothelial adhesion and mobilization of new unactivated cells may result in a net estimate of leukocyte activation not suspected to be positively correlated to acute-phase reactants. |
| Databáze: | OpenAIRE |
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