Electrical stimulation promotes the survival of oligodendrocytes in mixed cortical cultures
| Autor: | Devin S. Gary, Thierry Houdayer, Misti Malone, John W. McDonald, Paul Capestany |
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| Rok vydání: | 2011 |
| Předmět: |
Cell Survival
Biophysics Stimulation Cell Count Tetrodotoxin Biology Cysteine Proteinase Inhibitors Amino Acid Chloromethyl Ketones White matter Rats Sprague-Dawley Cellular and Molecular Neuroscience Myelin Pregnancy Glial Fibrillary Acidic Protein medicine Premovement neuronal activity Animals Axon Anesthetics Local Antigens Cells Cultured Cell Proliferation Cerebral Cortex Neurons Analysis of Variance Caspase 3 Sodium channel Stem Cells Cell Differentiation Myelin Basic Protein Oligodendrocyte Electric Stimulation Rats Oligodendroglia medicine.anatomical_structure Bromodeoxyuridine Phosphopyruvate Hydratase Female Proteoglycans Neuron Neuroscience Galactosylceramidase |
| Zdroj: | Journal of neuroscience research. 90(1) |
| ISSN: | 1097-4547 |
| Popis: | Oligodendrocyte (OLG) death plays a major role in white matter dysfunction and demyelination following injury to the CNS. Axonal contact, communication, and neuronal activity appear to promote OLG survival and function in cell culture and during development. The application of electrical stimulation to mixed neural cultures has been shown to promote OLG differentiation and the formation of myelin in vitro. Here we show that OLG viability can be significantly enhanced in mixed cortical cultures by applying biphasic pulses of electrical stimulation (ESTIM). Enhanced survival via ESTIM requires the presence of neurons and is suppressed by inhibition of voltage-gated sodium channels. Additionally, contact between the axon and OLG is necessary for ESTIM to promote OLG survival. This report suggests that patterned neuronal activity could repress delayed progression of white matter injury and promote CNS repair in neurological conditions that involve white matter damage. Journal of Neuroscience Research (2011) © 2011 Wiley Periodicals, Inc. |
| Databáze: | OpenAIRE |
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