In vitro vasoactive effects of dexmedetomidine on isolated human umbilical arteries
| Autor: | Bahar Oc, S B Taylan, Çetin Çelik, Hulagu Bariskaner, A Tekin, Setenay Arzu Yılmaz, Ipek Duman, J B Celik, Oguzhan Arun |
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| Přispěvatelé: | Selçuk Üniversitesi, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü, Arun, O., Taylan, S. B., Oc, B., Yilmaz, S. A., Tekin, A., Celik, C., Bariskaner H., Celik J. B. |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Economics and Econometrics
alpha adrenergic receptors Propranolol Pharmacology In Vitro Techniques Muscle Smooth Vascular Umbilical Arteries 03 medical and health sciences 0302 clinical medicine 030202 anesthesiology medicine.artery Materials Chemistry Media Technology medicine Prazosin Humans Dexmedetomidine 030219 obstetrics & reproductive medicine Chemistry dexmedetomidine Forestry Umbilical artery in vitro Analgesics Non-Narcotic Yohimbine Atropine Vasoconstriction vascular smooth muscle umbilical cord Verapamil Calcium medicine.symptom medicine.drug Muscle Contraction |
| Popis: | WOS: 000457596700007 PubMed: 30685991 OBJECTIVE: We aimed to investigate the vasoactive effects of dexmedetomidine on isolated human umbilical arteries and possible mechanisms involved. METHODS: Human umbilical artery strips were suspended in Krebs-Henseleit solution and dose-response curves were obtained for cumulative dexmedetomidine before and after incubation with different agents; propranolol, atropine, yohimbine, prazosin, indomethacin, verapamil. Effects of calcium on cumulative dexmedetomidine-induced contractions were also studied. RESULTS: Cumulative dexmedetomidine resulted in dose dependent contraction responses. Incubation with propranolol (Emax: 93.3 +/- 3.26 %), atropine (Emax: 92.0 +/- 6.54 %), or indomethacin (Emax: 94.25 +/- 2.62 %), did not attenuate dexmedetomidine-elicited contractions (p > 0.05). There were significant decreases in the contraction responses of cumulative dexmedetomidine with yohimbine (Emax: 12.1 +/- 11.9 %), prazosin (Emax: 28.8 +/- 4.6 %) and verapamil (Emax: 11.2 +/- 13.6 %) (p < 0.05). In Ca+2 free medium contraction responses to cumulative dexmedetomidine was insignificant (Emax: 5.20 +/- 3.42 %). Addition of cumulative calcium to the Ca+2 free medium resulted in concentration dependent increase in contractions (Emax: 64.83 +/- 37.7 %) (p < 0.05). CONCLUSION: Dexmedetomidine induces vasoconstriction in endothelial-free umbilical arteries via both, alpha(1)- and alpha(2)-adrenergic receptors and also extracellular Ca+2 concentrations play a major role. beta-adrenergic receptors, muscarinic cholinergic receptors, and inhibition of cyclooxygenase enzyme are not involved in this vasoconstriction (Fig. 3, Ref. 36). Text in PDF www.elis.sk. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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