Detection of Hepatitis E virus in shellfish harvesting areas from Galicia (Northwestern Spain)
| Autor: | Jesús L. Romalde, Maria São José Nascimento, Enrique Rivadulla, Miguel F. Varela, João R. Mesquita |
|---|---|
| Přispěvatelé: | Universidade de Santiago de Compostela. Departamento de Microbioloxía e Parasitoloxía |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Veterinary medicine Genotyping Genotype viruses 030106 microbiology Population lcsh:QR1-502 detection hepatitis E virus Biology medicine.disease_cause Virus lcsh:Microbiology Article law.invention Food safety 03 medical and health sciences Hepatitis E virus law Virology medicine Prevalence Animals Humans education Shellfish Phylogeny education.field_of_study Phylogenetic tree Sequence Analysis RNA virus diseases food and beverages Bivalvia shellfish food safety Detection 030104 developmental biology Infectious Diseases Transmission (mechanics) genotyping Spain Food Microbiology RNA Viral |
| Zdroj: | Viruses Volume 11 Issue 7 Viruses, Vol 11, Iss 7, p 618 (2019) Minerva. Repositorio Institucional de la Universidad de Santiago de Compostela instname |
| Popis: | The hepatitis E virus (HEV) affects almost 20 million individuals annually, causing approximately 3.3 million acute liver injuries, 56,600 deaths, and huge healthcare-associated economic losses. Shellfish produced close to urban and livestock areas can bioaccumulate this virus and transmit it to the human population. The aim of this study was to evaluate the presence of HEV in molluscan shellfish, in order to deepen the knowledge about HEV prevalence in Galicia (northwestern Spain), and to investigate this as a possible route of HEV transmission to humans. A total of 168 shellfish samples was obtained from two different Galician rí as (Rí a de Ares-Betanzos and Rí a de Vigo). The samples were analyzed by reverse transcription-quantitative PCR (RT-qPCR). RT-nested PCR and sequencing were used for further genotyping and phylogenetic analysis of positive samples. HEV was detected in 41 (24.4%) samples, at quantification levels ranging from non-quantifiable (< 102 copies of the RNA genome (RNAc)/g tissue) to 1.1 × 105 RNAc/g tissue. Phylogenetic analysis based on the open reading frame (ORF)2 region showed that all sequenced isolates belonged to genotype 3, and were closely related to strains of sub-genotype e, which is of swine origin. The obtained results demonstrate a significant prevalence of HEV in bivalve molluscs from Galician rí as, reinforcing the hypothesis that shellfish may be a potential route for HEV transmission to humans. |
| Databáze: | OpenAIRE |
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