A Multicenter Analysis of Subjectivity of Indirect Immunofluorescence Test in Antinuclear Antibody Screening
| Autor: | Vildan Turan Faraşat, Yavuz Doğan, Gülfem Terek Ece, Tamer Şanlidağ, Talat Ecemiş, Pınar Erbay Dündar, Aslı Gamze Şener |
|---|---|
| Přispěvatelé: | Department of Medical Microbiology, Manisa Celal Bayar University, Faculty of Medicine, Manisa, Turkey, Department of Medical Microbiology, Dokuz Eylül University, Faculty of Medicine, Izmir, Turkey, Department of Medical Microbiology, Katip Çelebi University, Faculty of Medicine, Izmir, Turkey, Department of Medical Microbiology, Izmir Medicalpark Hospital, Izmir, Turkey, Department of Public Health, Manisa Celal Bayar University, Faculty of Medicine, Manisa, Turkey |
| Rok vydání: | 2019 |
| Předmět: |
030203 arthritis & rheumatology
medicine.medical_specialty Indirect immunofluorescence Anti-nuclear antibody business.industry Gold standard (test) Predictive value Gastroenterology 03 medical and health sciences Fluorescence intensity 0302 clinical medicine Rheumatology Internal medicine Medicine Original Article 030212 general & internal medicine Positive test business |
| Zdroj: | Archives of Rheumatology. 34:326-333 |
| ISSN: | 2618-6500 2148-5046 |
| DOI: | 10.5606/archrheumatol.2019.7310 |
| Popis: | Objectives: This study aims to evaluate the interpretation of the antinuclear antibody (ANA)-indirect immunofluorescence (IIF) test results based on the interpreter-related subjectivity and to examine the inter-center agreement rates with the performance of each laboratory. Patients and methods: The ANA-IIF testing was carried out in a total of 600 sera and evaluated by four laboratories. The inter-center agreement rates were detected. The same results given by the four centers were accepted as gold standard and the predictive values of each center were calculated. Results: The inter-center agreement was reported for ANA-IIF test results from 392 of 600 (65.3%) sera, while 154 of 392 results were positive. Four study centers reported 213 (35.5%), 222 (37.0%), 266 (44.3%), and 361 (60.2%) positive test results, respectively. In terms of the patterns, the highest and lowest positive predictive values were 72.3% and 42.7%, respectively, while the highest and lowest negative predictive values were 99.6% and 61.5%, respectively. The agreement for semi-quantitative evaluation at three levels of fluorescence intensity stated by four centers was detected in 100 sera at 87% 3(+), while the other two levels were 6% and 7%. The highest predictive value for the highest fluorescence intensity of 3(+) was found to be 71.9%. Conclusion: Significant differences may be observed among laboratories in terms of qualitative results, patterns, and semi-quantitative determination of the fluorescence intensity in the ANA-IIF testing, particularly at low fluorescence intensity levels and in those with speckled patterns. In case of any discrepancy between ANA-IIF test and clinical prediagnosis, the test should be repeated in another laboratory, if necessary. © 2019 Turkish League Against Rheumatism. |
| Databáze: | OpenAIRE |
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