Decreased integrity of exercise-induced plasma cell free nuclear DNA – negative association with the increased oxidants production by circulating phagocytes
| Autor: | Tomasz Budlewski, Piotr Kosielski, Gianluca Padula, Pawel Meissner, Konrad Walczak, Anna Wlodarczyk, Agata Sarniak, Robert Stawski, Dariusz Nowak, Ewelina Perdas |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Mitochondrial DNA Phagocyte lcsh:Medicine Negative association Plasma cell Article 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Internal medicine medicine DNA metabolism Author Correction lcsh:Science Whole blood chemistry.chemical_classification Reactive oxygen species Multidisciplinary lcsh:R 030229 sport sciences Circulating Cell-Free DNA Nuclear DNA 030104 developmental biology Endocrinology medicine.anatomical_structure chemistry lcsh:Q human activities Biological fluorescence DNA |
| Zdroj: | Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-11 (2019) |
| ISSN: | 2045-2322 |
| Popis: | Objective Strenuous exercise increases circulating cell free DNA (cf DNA) and stimulates blood phagocytes to generate more reactive oxygen species (ROS) which may induce DNA strand breaks. We tested whether: (A) elevated cf DNA in response to three repeated bouts of exhaustive exercise has decreased integrity; (B) each bout of exercise increases luminol enhanced whole blood chemiluminescence (LBCL) as a measure of ROS production by polymorphonuclear leukocytes; (C) there is an association between integrity of cf DNA and LBCL. Methods Eleven average-trained men performed three treadmill exercise tests to exhaustion at speed corresponding to 70% of their individual VO2max separated by 72 hours of resting. Pre- and post-exercise concentrations and integrity of cf nuclear and mitochondrial DNA (cf n-DNA, cf mt-DNA) were determined with real-time PCR along with measurement of resting and fMLP-stimulated LBCL. Results Each bout increased concentrations of cf n-DNA by more than 10-times which was accompanied by about 2-times elevated post-exercise rLBCL and fMLP-LBCL (p |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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