Prognostic significance of preoperative and postoperative CK19 and CEA mRNA levels in peripheral blood of patients with gastric cardia cancer

Autor: Zhao Ma, Chuan Gui Chen, Jie Yue, Ming Quan Ma, Yu Feng Qiao, Zhen Tao Yu
Rok vydání: 2017
Předmět:
Male
Oncology
Cea mrna
Kaplan-Meier Estimate
Gastroenterology
0302 clinical medicine
Carcinoembryonic antigen
Medicine
Aged
80 and over
biology
Cardia
General Medicine
Middle Aged
Prognosis
Gene Expression Regulation
Neoplastic
Treatment Outcome
Lymphatic Metastasis
030220 oncology & carcinogenesis
Female
030211 gastroenterology & hepatology
Gastric cardia cancer
Adult
medicine.medical_specialty
digestive system
03 medical and health sciences
Stomach Neoplasms
Retrospective Study
Internal medicine
Biomarkers
Tumor
otorhinolaryngologic diseases
Gastric Cardia Cancer
Humans
Clinicopathological factor
RNA
Messenger
neoplasms
Aged
Proportional Hazards Models
Retrospective Studies
Keratin-19
business.industry
Cytokeratin 19
digestive system diseases
Peripheral blood
Carcinoembryonic Antigen
ROC Curve
Multivariate Analysis
biology.protein
business
Follow-Up Studies
Zdroj: World Journal of Gastroenterology
ISSN: 1007-9327
Popis: AIM To evaluate the clinical and prognostic significance of preoperative and postoperative cytokeratin 19 (CK19) and carcinoembryonic antigen (CEA) mRNA levels in peripheral blood of patients with gastric cardia cancer (GCC). METHODS We detected the preoperative and postoperative mRNA levels of CK19 and CEA in peripheral blood of 129 GCC patients by using reverse transcription-polymerase chain reaction and evaluated their clinical and prognostic significance by univariate Kaplan-Meier survival analysis and multivariate Cox proportional hazard analysis. A new prognostic model which stratified patients into three different risk groups was established based on the independent prognostic factors. RESULTS Elevated preoperative and postoperative CK19 and CEA mRNA levels in peripheral blood of GCC patients were associated with lymph node metastasis. Univariate analysis showed that tumor size, histological grade, depth of tumor invasion, lymph node metastasis, preoperative CK19 mRNA, and preoperative and postoperative CEA mRNA levels were correlated with the prognosis of GCC patients. The multivariate analysis showed that lymph node status (P = 0.018), preoperative CK19 (P = 0.035) and CEA (P = 0.011) mRNA levels were independent prognostic factors for overall survival (OS). The 5-year OS rates for the low-, intermediate-, and high-risk groups were 48.3%, 22.6%, and 4.6%, respectively (P < 0.001). CONCLUSION Elevated preoperative CK19 and CEA mRNA levels may be regarded as promising biomarkers for predicting lymph node metastasis and poor prognosis in patients with GCC. This new prognostic model may help us identify the subpopulations of GCC patients with the highest risk.
Databáze: OpenAIRE
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