Regulation of Proliferation and Epithelial-to-Mesenchymal Transition (EMT) of Gastric Cancer by ZEB1 via Modulating Wnt5a and Related Mechanisms
| Autor: | Ligong Zhang, Yongju Xue, Xiquan Ke, Yu Zhu, Haiyang Min, Qizhi Wang |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Adult
Male Epithelial-Mesenchymal Transition Down-Regulation Apoptosis Vimentin 030204 cardiovascular system & hematology Wnt-5a Protein Flow cytometry 03 medical and health sciences 0302 clinical medicine Re-Epithelialization Downregulation and upregulation Cell Movement Stomach Neoplasms Clinical Research Cell Line Tumor medicine Humans Epithelial–mesenchymal transition RNA Small Interfering Aged Cell Proliferation Homeodomain Proteins biology medicine.diagnostic_test Cell growth Chemistry Zinc Finger E-box-Binding Homeobox 1 Cell migration General Medicine Transfection Middle Aged Up-Regulation Cell culture 030220 oncology & carcinogenesis biology.protein Cancer research Female Transcription Factors |
| Zdroj: | Medical Science Monitor : International Medical Journal of Experimental and Clinical Research |
| ISSN: | 1643-3750 |
| DOI: | 10.12659/msm.912338 |
| Popis: | BACKGROUND As a member of the zinc-finger E-box binding protein (ZEB) family, ZEB1 can modulate onset and progression of various tumors, but its regulatory effect or mechanism in GC has not been defined. MATERIAL AND METHODS GC tumor tissues and adjacent tissues were collected from GC patients across different TNM stages. Real-time PCR was used to measure ZEB1 expression to analyze its correlation with pathological features of tumors. Cultured GC cell lines SGC-7901 and MGC-803 were randomly assigned into control group, scramble group, and ZEB1 siRNA group. Real-time PCR was employed to analyze ZEB1 expression, and MTT approach was used to measure cell proliferation. Cell apoptosis was evaluated by flow cytometry. Wound healing assay was used to detect its effect on cell migration. Expression of E-cadherin and Vimentin involved in epithelial-to-mesenchymal transition (EMT) was measured by Western blot analysis, along with Wnt5a proteins. RESULTS GC tissues had upregulation of ZEB1 (P |
| Databáze: | OpenAIRE |
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